Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              17    4312
XP_006714104                                                           17    4312
XP_005265800                                                           17    4312
XP_006714103                                                           17    4312
XP_006714102                                                           17    4312
NP_072093                                                              16    7355
NP_072179                                                              15    9605
NP_002881                                                              15    9605

>NP_006197
          Length = 1089

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           VAA VLVLL
Sbjct: 527 VAAAVLVLL 535



>XP_006714104
          Length = 832

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           VAA VLVLL
Sbjct: 552 VAAAVLVLL 560



>XP_005265800
          Length = 1089

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           VAA VLVLL
Sbjct: 527 VAAAVLVLL 535



>XP_006714103
          Length = 1111

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           VAA VLVLL
Sbjct: 549 VAAAVLVLL 557



>XP_006714102
          Length = 1114

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           VAA VLVLL
Sbjct: 552 VAAAVLVLL 560



>NP_072093
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           VAA+ LVLL
Sbjct: 103 VAAQALVLL 111



>NP_072179
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           +AAR L+L+
Sbjct: 748 IAARTLILV 756



>NP_002881
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VAARVLVLL 9
           +AAR L+L+
Sbjct: 925 IAARTLILV 933



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.154    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 218,197
Number of extensions: 152
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)