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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006545                                                              18    1935
NP_005742                                                              17    2528
NP_671714                                                              17    2528
XP_006715890                                                           17    2528
XP_005250160                                                           17    2528
XP_005250159                                                           17    2528
NP_001172026                                                           15    9605
NP_000198                                                              15    9605
NP_001172027                                                           15    9605
NP_001035835                                                           15    9605

>NP_006545
          Length = 263

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VAAAFVSQI 9
          VA AFVSQI
Sbjct: 4  VAEAFVSQI 12



>NP_005742
          Length = 3907

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAAAFVSQI 9
            VAAA VSQI
Sbjct: 2551 VAAALVSQI 2559



>NP_671714
          Length = 3899

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAAAFVSQI 9
            VAAA VSQI
Sbjct: 2543 VAAALVSQI 2551



>XP_006715890
          Length = 3860

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAAAFVSQI 9
            VAAA VSQI
Sbjct: 2504 VAAALVSQI 2512



>XP_005250160
          Length = 3939

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAAAFVSQI 9
            VAAA VSQI
Sbjct: 2583 VAAALVSQI 2591



>XP_005250159
          Length = 3952

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAAAFVSQI 9
            VAAA VSQI
Sbjct: 2596 VAAALVSQI 2604



>NP_001172026
          Length = 110

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AAAFVSQ 8
          AAAFV+Q
Sbjct: 22 AAAFVNQ 28



>NP_000198
          Length = 110

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AAAFVSQ 8
          AAAFV+Q
Sbjct: 22 AAAFVNQ 28



>NP_001172027
          Length = 110

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AAAFVSQ 8
          AAAFV+Q
Sbjct: 22 AAAFVNQ 28



>NP_001035835
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AAAFVSQ 8
          AAAFV+Q
Sbjct: 22 AAAFVNQ 28



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.129    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 249,992
Number of extensions: 147
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)