Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_705833                                                              18    1935
XP_005247174                                                           18    1935
NP_005304                                                              17    3301
NP_057407                                                              16    5631
XP_006712935                                                           16    5631
XP_006712934                                                           16    5631
XP_006712933                                                           16    5631
NP_852118                                                              16    5631
NP_001073946                                                           16    7355
NP_001258593                                                           16    7355
NP_079229                                                              16    7355
NP_001269076                                                           16    7355
NP_001001413                                                           16    7355
NP_060296                                                              16    7355
NP_001185839                                                           16    7355
XP_006726624                                                           16    7355
XP_006726623                                                           16    7355
XP_006725630                                                           16    7355
XP_006725629                                                           16    7355
XP_006725599                                                           16    7355
XP_006726291                                                           16    7355
XP_006726290                                                           16    7355
XP_006725238                                                           16    7355
XP_006720863                                                           16    7355
XP_006720862                                                           16    7355
XP_006720860                                                           16    7355
XP_006720859                                                           16    7355
XP_006720858                                                           16    7355
XP_006720426                                                           16    7355
XP_005253995                                                           16    7355
XP_006719669                                                           16    7355
XP_006714321                                                           16    7355
XP_006714320                                                           16    7355
XP_006714319                                                           16    7355
NP_114091                                                              15    9605
NP_002664                                                              15    9605
NP_071920                                                              15    9605
NP_001123554                                                           15    9605
NP_060252                                                              15    9605
NP_071505                                                              15    9605
NP_071320                                                              15    9605
NP_525023                                                              15    9605
NP_115812                                                              15    9605
NP_075463                                                              15    9605
NP_001157617                                                           15    9605
NP_001157616                                                           15    9605
XP_006721856                                                           15    9605
XP_006721855                                                           15    9605
XP_006721854                                                           15    9605
XP_006721853                                                           15    9605
XP_005256139                                                           15    9605
XP_005256138                                                           15    9605
XP_005256137                                                           15    9605
XP_005266254                                                           15    9605
XP_006719812                                                           15    9605
XP_005252540                                                           15    9605
XP_005252539                                                           15    9605
XP_005252538                                                           15    9605
XP_005252537                                                           15    9605
XP_005252535                                                           15    9605
XP_005252534                                                           15    9605
XP_006717515                                                           15    9605
XP_005252533                                                           15    9605
XP_005252532                                                           15    9605
XP_005252531                                                           15    9605
XP_006714351                                                           15    9605
XP_005263235                                                           15    9605
XP_006713269                                                           15    9605
XP_005264590                                                           15    9605
XP_005264589                                                           15    9605
XP_005264588                                                           15    9605
XP_005264587                                                           15    9605
XP_005264585                                                           15    9605
XP_005264584                                                           15    9605
XP_005264583                                                           15    9605
XP_005264582                                                           15    9605
XP_005264581                                                           15    9605
>NP_705833
          Length = 2970

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    TYEKNAK 7
            TY+KNAK
Sbjct: 1765 TYQKNAK 1771
>XP_005247174
          Length = 2679

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    TYEKNAK 7
            TY+KNAK
Sbjct: 1474 TYQKNAK 1480
>NP_005304
          Length = 505

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   YEKNAK 7
           YEKNAK
Sbjct: 269 YEKNAK 274
>NP_057407
          Length = 1024

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TYEKNAK 7
           T+EKNA+
Sbjct: 919 TFEKNAR 925
>XP_006712935
          Length = 977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           YE+N KLS
Sbjct: 694 YEENNKLS 701
>XP_006712934
          Length = 1583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           YE+N KLS
Sbjct: 590 YEENNKLS 597
>XP_006712933
          Length = 1687

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           YE+N KLS
Sbjct: 694 YEENNKLS 701
>NP_852118
          Length = 1684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           YE+N KLS
Sbjct: 691 YEENNKLS 698
>NP_001073946
          Length = 2699

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    YEKNAKLS 9
            Y K+AKLS
Sbjct: 1465 YAKDAKLS 1472
>NP_001258593
          Length = 670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 209 EKNAKL 214
>NP_079229
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TYEKNAKLS 9
           T +KN KLS
Sbjct: 429 TMDKNQKLS 437
>NP_001269076
          Length = 225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           +EKN K+S
Sbjct: 196 FEKNKKIS 203
>NP_001001413
          Length = 668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 221 EKNAKL 226
>NP_060296
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           +EKN K+S
Sbjct: 196 FEKNKKIS 203
>NP_001185839
          Length = 502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YEKNAKLS 9
           +EKN K+S
Sbjct: 273 FEKNKKIS 280
>XP_006726624
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006726623
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006725630
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006725629
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006725599
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 221 EKNAKL 226
>XP_006726291
          Length = 624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006726290
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006725238
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 221 EKNAKL 226
>XP_006720863
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 203 EKNAKL 208
>XP_006720862
          Length = 570

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 209 EKNAKL 214
>XP_006720860
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 197 EKNAKL 202
>XP_006720859
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 197 EKNAKL 202
>XP_006720858
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 197 EKNAKL 202
>XP_006720426
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EKNAKL 8
           EKNAKL
Sbjct: 221 EKNAKL 226
>XP_005253995
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TYEKNAKLS 9
           T +KN KLS
Sbjct: 293 TMDKNQKLS 301
>XP_006719669
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TYEKNAKLS 9
           T +KN KLS
Sbjct: 401 TMDKNQKLS 409
>XP_006714321
          Length = 2713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    YEKNAKLS 9
            Y K+AKLS
Sbjct: 1479 YAKDAKLS 1486
>XP_006714320
          Length = 2720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    YEKNAKLS 9
            Y K+AKLS
Sbjct: 1486 YAKDAKLS 1493
>XP_006714319
          Length = 2721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    YEKNAKLS 9
            Y K+AKLS
Sbjct: 1487 YAKDAKLS 1494
>NP_114091
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YEKNAK 7
           YE+NAK
Sbjct: 355 YEENAK 360
>NP_002664
          Length = 2135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TYEKNAKLS 9
           +YE+ AKL+
Sbjct: 199 SYEETAKLA 207
>NP_071920
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   YEKNAKL 8
           Y+KN KL
Sbjct: 293 YKKNGKL 299
>NP_001123554
          Length = 2135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TYEKNAKLS 9
           +YE+ AKL+
Sbjct: 199 SYEETAKLA 207
>NP_060252
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>NP_071505
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 192 TYESNTK 198
>NP_071320
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 181 TYESNTK 187
>NP_525023
          Length = 1617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    YEKNAK 7
            Y+KNAK
Sbjct: 1039 YKKNAK 1044
>NP_115812
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  YEKNAKL 8
          Y+K+AKL
Sbjct: 55 YQKSAKL 61
>NP_075463
          Length = 3013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    EKNAKLS 9
            EKN KLS
Sbjct: 2177 EKNPKLS 2183
>NP_001157617
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  YEKNAKL 8
          Y+K+AKL
Sbjct: 55 YQKSAKL 61
>NP_001157616
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  YEKNAKL 8
          Y+K+AKL
Sbjct: 55 YQKSAKL 61
>XP_006721856
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YEKNAK 7
           Y+KNAK
Sbjct: 722 YKKNAK 727
>XP_006721855
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    YEKNAK 7
            Y+KNAK
Sbjct: 1039 YKKNAK 1044
>XP_006721854
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    YEKNAK 7
            Y+KNAK
Sbjct: 1039 YKKNAK 1044
>XP_006721853
          Length = 1617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    YEKNAK 7
            Y+KNAK
Sbjct: 1039 YKKNAK 1044
>XP_005256139
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YEKNAK 7
           YE+NAK
Sbjct: 355 YEENAK 360
>XP_005256138
          Length = 510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YEKNAK 7
           YE+NAK
Sbjct: 355 YEENAK 360
>XP_005256137
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YEKNAK 7
           YE+NAK
Sbjct: 355 YEENAK 360
>XP_005266254
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  YEKNAKL 8
          Y+K+AKL
Sbjct: 55 YQKSAKL 61
>XP_006719812
          Length = 3019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    EKNAKLS 9
            EKN KLS
Sbjct: 2174 EKNPKLS 2180
>XP_005252540
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>XP_005252539
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>XP_005252538
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>XP_005252537
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>XP_005252535
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>XP_005252534
          Length = 2521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1146 TFEKNA 1151
>XP_006717515
          Length = 2521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1146 TFEKNA 1151
>XP_005252533
          Length = 2521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1146 TFEKNA 1151
>XP_005252532
          Length = 2430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1055 TFEKNA 1060
>XP_005252531
          Length = 2670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TYEKNA 6
            T+EKNA
Sbjct: 1295 TFEKNA 1300
>XP_006714351
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   YEKNAKL 8
           Y+KN KL
Sbjct: 293 YKKNGKL 299
>XP_005263235
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   YEKNAKL 8
           Y+KN KL
Sbjct: 293 YKKNGKL 299
>XP_006713269
          Length = 2052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TYEKNAKLS 9
           +YE+ AKL+
Sbjct: 116 SYEETAKLA 124
>XP_005264590
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1  TYEKNAK 7
          TYE N K
Sbjct: 52 TYESNTK 58
>XP_005264589
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1  TYEKNAK 7
          TYE N K
Sbjct: 91 TYESNTK 97
>XP_005264588
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1  TYEKNAK 7
          TYE N K
Sbjct: 91 TYESNTK 97
>XP_005264587
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1  TYEKNAK 7
          TYE N K
Sbjct: 91 TYESNTK 97
>XP_005264585
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 127 TYESNTK 133
>XP_005264584
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 181 TYESNTK 187
>XP_005264583
          Length = 377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 183 TYESNTK 189
>XP_005264582
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 194 TYESNTK 200
>XP_005264581
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   TYEKNAK 7
           TYE N K
Sbjct: 194 TYESNTK 200
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.109    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 486,865
Number of extensions: 661
Number of successful extensions: 77
Number of sequences better than 10000.0: 77
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 77
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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