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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              18    1135
NP_066124                                                              18    1135
XP_006717999                                                           18    1135
NP_277040                                                              17    2528
NP_079441                                                              17    3301
NP_997698                                                              17    4312
NP_001597                                                              17    4312
XP_006717059                                                           17    4312
XP_005273301                                                           16    7355
NP_003263                                                              16    7355
NP_001035706                                                           15    9605
NP_001004334                                                           15    9605
NP_001186948                                                           15    9605
NP_001010986                                                           15    9605
NP_775965                                                              15    9605
NP_872588                                                              15    9605
NP_000904                                                              15    9605
XP_006724816                                                           15    9605
XP_006724815                                                           15    9605
XP_006724814                                                           15    9605
XP_005262462                                                           15    9605
XP_006723863                                                           15    9605
XP_005260263                                                           15    9605
XP_006723862                                                           15    9605
XP_005255000                                                           15    9605
XP_005254998                                                           15    9605
NP_001123475                                                           15    9605
NP_653243                                                              15    9605

>NP_065681
          Length = 1072

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VLFSFIVS 9
           VLFSFIVS
Sbjct: 642 VLFSFIVS 649



>NP_066124
          Length = 1114

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VLFSFIVS 9
           VLFSFIVS
Sbjct: 642 VLFSFIVS 649



>XP_006717999
          Length = 1207

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VLFSFIVS 9
           VLFSFIVS
Sbjct: 735 VLFSFIVS 742



>NP_277040
          Length = 397

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   VLFSFIVS 9
           VLFSFI+S
Sbjct: 163 VLFSFILS 170



>NP_079441
          Length = 397

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  LFSFIVS 9
          LFSFIVS
Sbjct: 68 LFSFIVS 74



>NP_997698
          Length = 2466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TVLFSFIVS 9
           T++F F+VS
Sbjct: 827 TIMFCFLVS 835



>NP_001597
          Length = 2436

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TVLFSFIVS 9
           T++F F+VS
Sbjct: 797 TIMFCFLVS 805



>XP_006717059
          Length = 2435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TVLFSFIVS 9
           T++F F+VS
Sbjct: 796 TIMFCFLVS 804



>XP_005273301
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  TVLFSF 6
          TVLFSF
Sbjct: 93 TVLFSF 98



>NP_003263
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  TVLFSF 6
          TVLFSF
Sbjct: 93 TVLFSF 98



>NP_001035706
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TVLFSFIVS 9
           TVL  F++S
Sbjct: 507 TVLLDFLIS 515



>NP_001004334
          Length = 2367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TVLFSFIV 8
           TVLF F++
Sbjct: 421 TVLFGFLL 428



>NP_001186948
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LFSFIV 8
           LFSFIV
Sbjct: 221 LFSFIV 226



>NP_001010986
          Length = 1119

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLFSFIV 8
           VLF+FI+
Sbjct: 352 VLFNFII 358



>NP_775965
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLFSFIV 8
           VLF+FI+
Sbjct: 352 VLFNFII 358



>NP_872588
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LFSFIV 8
           LFSFIV
Sbjct: 221 LFSFIV 226



>NP_000904
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LFSFIV 8
           LFSFIV
Sbjct: 221 LFSFIV 226



>XP_006724816
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLFSFIV 8
           VLF+FI+
Sbjct: 349 VLFNFII 355



>XP_006724815
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLFSFIV 8
           VLF+FI+
Sbjct: 349 VLFNFII 355



>XP_006724814
          Length = 1116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLFSFIV 8
           VLF+FI+
Sbjct: 349 VLFNFII 355



>XP_005262462
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLFSFIV 8
           VLF+FI+
Sbjct: 349 VLFNFII 355



>XP_006723863
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LFSFIV 8
           LFSFIV
Sbjct: 140 LFSFIV 145



>XP_005260263
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LFSFIV 8
           LFSFIV
Sbjct: 216 LFSFIV 221



>XP_006723862
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LFSFIV 8
           LFSFIV
Sbjct: 221 LFSFIV 226



>XP_005255000
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TVLFSFIVS 9
           TVL  F++S
Sbjct: 471 TVLLDFLIS 479



>XP_005254998
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TVLFSFIVS 9
           TVL  F++S
Sbjct: 507 TVLLDFLIS 515



>NP_001123475
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TVLFSFI 7
           TV+FSF+
Sbjct: 141 TVVFSFL 147



>NP_653243
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TVLFSFI 7
           TV+FSF+
Sbjct: 121 TVVFSFL 127



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.149    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 342,963
Number of extensions: 613
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)