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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_056197                                                              16    7355
XP_005267560                                                           16    7355
XP_005267559                                                           16    7355
NP_001271282                                                           15    9605
NP_001271281                                                           15    9605
NP_065826                                                              15    9605
NP_001253977                                                           15    9605
NP_005399                                                              15    9605

>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TVFTSKKVS 9
            T FTSKKVS
Sbjct: 1521 TAFTSKKVS 1529



>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TVFTSKKVS 9
            T FTSKKVS
Sbjct: 1316 TAFTSKKVS 1324



>NP_056197
          Length = 2610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    FTSKKVS 9
            FTSKK++
Sbjct: 2036 FTSKKIT 2042



>XP_005267560
          Length = 2563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    FTSKKVS 9
            FTSKK++
Sbjct: 1989 FTSKKIT 1995



>XP_005267559
          Length = 2610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    FTSKKVS 9
            FTSKK++
Sbjct: 2036 FTSKKIT 2042



>NP_001271282
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VFTSKK 7
           +FTSKK
Sbjct: 636 IFTSKK 641



>NP_001271281
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VFTSKK 7
           +FTSKK
Sbjct: 872 IFTSKK 877



>NP_065826
          Length = 1013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VFTSKK 7
           +FTSKK
Sbjct: 974 IFTSKK 979



>NP_001253977
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VFTSKK 7
           +FTSKK
Sbjct: 887 IFTSKK 892



>NP_005399
          Length = 98

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FTSKKVS 9
          F+SKK+S
Sbjct: 38 FSSKKIS 44



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.113    0.268 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,611
Number of extensions: 342
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)