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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           18    1935
NP_001257437                                                           18    1935
NP_006281                                                              18    1935
XP_006715618                                                           18    1935
XP_005267176                                                           18    1935
NP_001106849                                                           15    9605
NP_060663                                                              15    9605
NP_001258636                                                           15    9605
NP_054879                                                              15    9605
NP_689845                                                              15    9605

>NP_001257436
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TVEASSSLS 9
           T EASSSLS
Sbjct: 544 TAEASSSLS 552



>NP_001257437
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TVEASSSLS 9
           T EASSSLS
Sbjct: 544 TAEASSSLS 552



>NP_006281
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TVEASSSLS 9
           T EASSSLS
Sbjct: 544 TAEASSSLS 552



>XP_006715618
          Length = 577

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TVEASSSLS 9
           T EASSSLS
Sbjct: 331 TAEASSSLS 339



>XP_005267176
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TVEASSSLS 9
           T EASSSLS
Sbjct: 544 TAEASSSLS 552



>NP_001106849
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TVEASSSLS 9
           T+E S SLS
Sbjct: 403 TIETSPSLS 411



>NP_060663
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TVEASSSLS 9
           T+E S SLS
Sbjct: 403 TIETSPSLS 411



>NP_001258636
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VEASSSLS 9
           VEASSS S
Sbjct: 115 VEASSSYS 122



>NP_054879
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VEASSSLS 9
           VEASSS S
Sbjct: 158 VEASSSYS 165



>NP_689845
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TVEASSSL 8
           TVEA SSL
Sbjct: 906 TVEAYSSL 913



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.272   0.0868    0.170 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 278,582
Number of extensions: 187
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.5 bits)
S2: 28 (15.4 bits)