Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_071371                                                              16    5631
NP_060667                                                              16    5631
NP_955458                                                              16    5631
XP_006723884                                                           16    5631
NP_955459                                                              16    5631
XP_006715518                                                           16    7355
XP_006715517                                                           16    7355
NP_066933                                                              15    9605
NP_775740                                                              15    9605
NP_001165419                                                           15    9605
NP_076974                                                              15    9605
NP_001138807                                                           15    9605
NP_001271311                                                           15    9605
NP_001165420                                                           15    9605
XP_006722946                                                           15    9605
XP_005259634                                                           15    9605
XP_006720833                                                           15    9605
XP_005254845                                                           15    9605
XP_005254844                                                           15    9605
XP_005251116                                                           15    9605
XP_006716714                                                           15    9605
>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TTTEMLTSE 9
            T TEMLTSE
Sbjct: 1567 TATEMLTSE 1575
>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TTTEMLTSE 9
            T TEMLTSE
Sbjct: 1469 TATEMLTSE 1477
>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TTTEMLTSE 9
            T TEMLTSE
Sbjct: 1462 TATEMLTSE 1470
>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TTTEMLTSE 9
            T TEMLTSE
Sbjct: 1469 TATEMLTSE 1477
>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TTTEMLTSE 9
            T TEMLTSE
Sbjct: 1567 TATEMLTSE 1575
>NP_071371
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TTTEMLTSE 9
          TTT  +TSE
Sbjct: 82 TTTRTITSE 90
>NP_060667
          Length = 681

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TTTEMLTSE 9
          TTT  +TSE
Sbjct: 82 TTTRTITSE 90
>NP_955458
          Length = 679

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TTTEMLTSE 9
          TTT  +TSE
Sbjct: 80 TTTRTITSE 88
>XP_006723884
          Length = 625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TTTEMLTSE 9
          TTT  +TSE
Sbjct: 80 TTTRTITSE 88
>NP_955459
          Length = 645

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TTTEMLTSE 9
          TTT  +TSE
Sbjct: 82 TTTRTITSE 90
>XP_006715518
          Length = 1338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    TTTEMLTSE 9
            ++TE+LTS+
Sbjct: 1006 SSTELLTSD 1014
>XP_006715517
          Length = 1379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    TTTEMLTSE 9
            ++TE+LTS+
Sbjct: 1006 SSTELLTSD 1014
>NP_066933
          Length = 1796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TEMLTSE 9
           TE++TSE
Sbjct: 357 TELITSE 363
>NP_775740
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTEMLTSE 9
           TTE +TSE
Sbjct: 132 TTEEVTSE 139
>NP_001165419
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTTEMLTSE 9
           TTT + TSE
Sbjct: 120 TTTTIATSE 128
>NP_076974
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTTEMLTSE 9
           TTT + TSE
Sbjct: 120 TTTTIATSE 128
>NP_001138807
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTEMLTSE 9
           TTE +TSE
Sbjct: 116 TTEEVTSE 123
>NP_001271311
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTEMLTSE 9
           TTE +TSE
Sbjct: 97  TTEEVTSE 104
>NP_001165420
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTTEMLTSE 9
           TTT + TSE
Sbjct: 120 TTTTIATSE 128
>XP_006722946
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTTEMLTSE 9
           TTT + TSE
Sbjct: 120 TTTTIATSE 128
>XP_005259634
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TTTEMLTS 8
           +TT MLTS
Sbjct: 232 STTAMLTS 239
>XP_006720833
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTEMLTSE 9
           TTE +TSE
Sbjct: 132 TTEEVTSE 139
>XP_005254845
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTEMLTSE 9
           TTE +TSE
Sbjct: 132 TTEEVTSE 139
>XP_005254844
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTEMLTSE 9
           TTE +TSE
Sbjct: 132 TTEEVTSE 139
>XP_005251116
          Length = 1780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TEMLTSE 9
           TE++TSE
Sbjct: 357 TELITSE 363
>XP_006716714
          Length = 1796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TEMLTSE 9
           TE++TSE
Sbjct: 357 TELITSE 363
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.285   0.0965    0.214 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,056
Number of extensions: 656
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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