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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000967                                                              17    4312
NP_001137452                                                           17    4312
NP_835465                                                              16    5631
XP_006721895                                                           16    5631
XP_006715638                                                           16    7355
XP_005267216                                                           16    7355
NP_115521                                                              16    7355

>NP_000967
          Length = 165

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RSLAREL 9
           RSLAREL
Sbjct: 119 RSLAREL 125



>NP_001137452
          Length = 667

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TRSLAREL 9
           TRSL REL
Sbjct: 97  TRSLLREL 104



>NP_835465
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  TRSLAREL 9
          TR+LAR+L
Sbjct: 8  TRALARQL 15



>XP_006721895
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  TRSLAREL 9
          TR+LAR+L
Sbjct: 8  TRALARQL 15



>XP_006715638
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TTRSLAREL 9
           TTRSLA  L
Sbjct: 189 TTRSLAAAL 197



>XP_005267216
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TTRSLAREL 9
           TTRSLA  L
Sbjct: 189 TTRSLAAAL 197



>NP_115521
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TTRSLAREL 9
           TTRSLA  L
Sbjct: 189 TTRSLAAAL 197



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.120    0.284 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,986
Number of extensions: 397
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)