Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              20     509
XP_005271621                                                           20     509
XP_006718907                                                           20     509
XP_006718906                                                           20     509
XP_005271619                                                           20     509
XP_005271618                                                           20     509
NP_001191273                                                           17    2528
NP_005109                                                              17    2528
NP_597702                                                              17    2528
NP_115642                                                              17    4312
NP_005038                                                              17    4312
NP_001257356                                                           17    4312
NP_001138502                                                           16    5631
NP_064613                                                              16    5631
NP_955469                                                              16    5631
NP_955471                                                              16    5631
NP_955470                                                              16    5631
NP_569120                                                              16    7355
NP_569122                                                              16    7355
NP_001387                                                              16    7355
NP_567824                                                              16    7355
NP_789763                                                              16    7355
NP_789762                                                              16    7355
NP_789764                                                              16    7355
NP_115488                                                              16    7355
XP_005260990                                                           16    7355
XP_005260988                                                           16    7355
XP_006724042                                                           16    7355
XP_006724041                                                           16    7355
XP_006724040                                                           16    7355
XP_006724039                                                           16    7355
XP_006718098                                                           16    7355
XP_005270288                                                           16    7355
XP_005270287                                                           16    7355
NP_075380                                                              15    9605
NP_872299                                                              15    9605
NP_006292                                                              15    9605
NP_001180440                                                           15    9605
NP_597705                                                              15    9605
NP_003765                                                              15    9605
NP_001186710                                                           15    9605
NP_001128128                                                           15    9605
NP_001186711                                                           15    9605
XP_005256292                                                           15    9605
XP_006721397                                                           15    9605
XP_005256291                                                           15    9605
XP_005256290                                                           15    9605
XP_006721396                                                           15    9605
XP_006719652                                                           15    9605
XP_006719651                                                           15    9605
XP_005269195                                                           15    9605
XP_006718578                                                           15    9605
XP_006718577                                                           15    9605
XP_006718574                                                           15    9605
XP_006718570                                                           15    9605
NP_872362                                                              15    9605
>NP_000042
          Length = 3056

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRGISSE
Sbjct: 491 TFRGISSE 498
>XP_005271621
          Length = 2708

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRGISSE
Sbjct: 143 TFRGISSE 150
>XP_006718907
          Length = 3050

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRGISSE
Sbjct: 491 TFRGISSE 498
>XP_006718906
          Length = 3056

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRGISSE
Sbjct: 491 TFRGISSE 498
>XP_005271619
          Length = 3056

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRGISSE
Sbjct: 491 TFRGISSE 498
>XP_005271618
          Length = 3056

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRGISSE
Sbjct: 491 TFRGISSE 498
>NP_001191273
          Length = 192

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRG++SE
Sbjct: 95  TFRGMTSE 102
>NP_005109
          Length = 251

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFRGISSE 9
           TFRG++SE
Sbjct: 154 TFRGMTSE 161
>NP_597702
          Length = 1115

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TTFRGIS 7
           TTFRG+S
Sbjct: 440 TTFRGLS 446
>NP_115642
          Length = 1984

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TTFRGISSE 9
            +TF GIS E
Sbjct: 1002 STFNGISQE 1010
>NP_005038
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FRGISSE 9
           FRGISS+
Sbjct: 399 FRGISSQ 405
>NP_001257356
          Length = 461

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FRGISSE 9
           FRGISS+
Sbjct: 356 FRGISSQ 362
>NP_001138502
          Length = 1692

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFRGISSE 9
           TFR +SSE
Sbjct: 828 TFRPVSSE 835
>NP_064613
          Length = 1160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TFRGISSE 9
            +FRG SSE
Sbjct: 1054 SFRGYSSE 1061
>NP_955469
          Length = 1156

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TFRGISSE 9
            +FRG SSE
Sbjct: 1050 SFRGYSSE 1057
>NP_955471
          Length = 1023

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFRGISSE 9
           +FRG SSE
Sbjct: 917 SFRGYSSE 924
>NP_955470
          Length = 1061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFRGISSE 9
           +FRG SSE
Sbjct: 955 SFRGYSSE 962
>NP_569120
          Length = 754

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 243 TNFRGVS 249
>NP_569122
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 252 TNFRGVS 258
>NP_001387
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 252 TNFRGVS 258
>NP_567824
          Length = 584

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 252 TNFRGVS 258
>NP_789763
          Length = 202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>NP_789762
          Length = 215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>NP_789764
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>NP_115488
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>XP_005260990
          Length = 535

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TTFRGIS 7
          T FRG+S
Sbjct: 24 TNFRGVS 30
>XP_005260988
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 223 TNFRGVS 229
>XP_006724042
          Length = 754

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 243 TNFRGVS 249
>XP_006724041
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 252 TNFRGVS 258
>XP_006724040
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 252 TNFRGVS 258
>XP_006724039
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TTFRGIS 7
           T FRG+S
Sbjct: 252 TNFRGVS 258
>XP_006718098
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>XP_005270288
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>XP_005270287
          Length = 164

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TTFRGI 6
           TTFRG+
Sbjct: 138 TTFRGL 143
>NP_075380
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FRGISSE 9
           FRG SSE
Sbjct: 278 FRGSSSE 284
>NP_872299
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TFRGISSE 9
           TF G+S E
Sbjct: 735 TFEGLSEE 742
>NP_006292
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 715 RGISSE 720
>NP_001180440
          Length = 892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 748 RGISSE 753
>NP_597705
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TTFRGISSE 9
            TTF  +SS+
Sbjct: 1048 TTFTAVSSD 1056
>NP_003765
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 451 RGISSE 456
>NP_001186710
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 448 RGISSE 453
>NP_001128128
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 106 RGISSE 111
>NP_001186711
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 279 RGISSE 284
>XP_005256292
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TFRGISSE 9
           TF G+S E
Sbjct: 394 TFEGLSEE 401
>XP_006721397
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TFRGISSE 9
           TF G+S E
Sbjct: 394 TFEGLSEE 401
>XP_005256291
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TFRGISSE 9
           TF G+S E
Sbjct: 594 TFEGLSEE 601
>XP_005256290
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TFRGISSE 9
           TF G+S E
Sbjct: 605 TFEGLSEE 612
>XP_006721396
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TFRGISSE 9
           TF G+S E
Sbjct: 735 TFEGLSEE 742
>XP_006719652
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 615 RGISSE 620
>XP_006719651
          Length = 892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 748 RGISSE 753
>XP_005269195
          Length = 892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGISSE 9
           RGISSE
Sbjct: 748 RGISSE 753
>XP_006718578
          Length = 150

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TTFRGI 6
          TT+RGI
Sbjct: 34 TTYRGI 39
>XP_006718577
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TTFRGI 6
          TT+RGI
Sbjct: 34 TTYRGI 39
>XP_006718574
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TTFRGI 6
          TT+RGI
Sbjct: 34 TTYRGI 39
>XP_006718570
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TTFRGI 6
          TT+RGI
Sbjct: 34 TTYRGI 39
>NP_872362
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TTFRGI 6
          TT+RGI
Sbjct: 34 TTYRGI 39
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.124    0.323 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 404,283
Number of extensions: 477
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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