Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     299
NP_001073289                                                           17    3301
NP_001120933                                                           17    3301
NP_899632                                                              17    3301
NP_001120934                                                           17    3301
NP_004886                                                              17    3301
NP_001230062                                                           17    3301
XP_005273095                                                           17    3301
XP_005273094                                                           17    3301
XP_006711796                                                           17    3301
XP_005273093                                                           17    3301
NP_570711                                                              17    4312
NP_570712                                                              17    4312
NP_570714                                                              17    4312
NP_570713                                                              17    4312
NP_570716                                                              17    4312
NP_000235                                                              17    4312
NP_570715                                                              17    4312
NP_004332                                                              17    4312
NP_071941                                                              17    4312
XP_006718620                                                           17    4312
XP_005274059                                                           17    4312
XP_005274060                                                           17    4312
XP_005274058                                                           17    4312
XP_005264615                                                           17    4312
XP_006712164                                                           17    4312
XP_005264614                                                           17    4312
XP_005264613                                                           17    4312
XP_005264612                                                           17    4312
NP_001207417                                                           16    5631
XP_006721183                                                           16    5631
NP_001248                                                              16    5631
NP_001207419                                                           16    5631
NP_001207418                                                           16    5631
NP_006222                                                              16    7355
NP_003092                                                              15    9605
NP_001239441                                                           15    9605
NP_002079                                                              15    9605
NP_001239440                                                           15    9605
NP_077015                                                              15    9605
XP_006723233                                                           15    9605
XP_006723232                                                           15    9605
XP_005258879                                                           15    9605
XP_005258878                                                           15    9605
NP_001244899                                                           15    9605
>NP_004295
          Length = 1620

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TSTIMIDYN 9
            TSTIM DYN
Sbjct: 1085 TSTIMTDYN 1093
>NP_001073289
          Length = 1036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>NP_001120933
          Length = 979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>NP_899632
          Length = 922

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>NP_001120934
          Length = 979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>NP_004886
          Length = 1036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>NP_001230062
          Length = 1034

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 53 ATLMIDFN 60
>XP_005273095
          Length = 979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>XP_005273094
          Length = 1036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>XP_006711796
          Length = 1036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>XP_005273093
          Length = 1036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STIMIDYN 9
          +T+MID+N
Sbjct: 55 ATLMIDFN 62
>NP_570711
          Length = 610

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 344 TATVIQDYN 352
>NP_570712
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 349 TATVIQDYN 357
>NP_570714
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 349 TATVIQDYN 357
>NP_570713
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 349 TATVIQDYN 357
>NP_570716
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 349 TATVIQDYN 357
>NP_000235
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 349 TATVIQDYN 357
>NP_570715
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 349 TATVIQDYN 357
>NP_004332
          Length = 2225

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIMIDYN 9
           TIM++YN
Sbjct: 969 TIMVNYN 975
>NP_071941
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   STIMIDY 8
           +T+MIDY
Sbjct: 230 NTVMIDY 236
>XP_006718620
          Length = 610

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 344 TATVIQDYN 352
>XP_005274059
          Length = 610

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 344 TATVIQDYN 352
>XP_005274060
          Length = 610

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 344 TATVIQDYN 352
>XP_005274058
          Length = 610

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSTIMIDYN 9
           T+T++ DYN
Sbjct: 344 TATVIQDYN 352
>XP_005264615
          Length = 2162

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIMIDYN 9
           TIM++YN
Sbjct: 906 TIMVNYN 912
>XP_006712164
          Length = 2179

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIMIDYN 9
           TIM++YN
Sbjct: 906 TIMVNYN 912
>XP_005264614
          Length = 2191

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIMIDYN 9
           TIM++YN
Sbjct: 969 TIMVNYN 975
>XP_005264613
          Length = 2208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIMIDYN 9
           TIM++YN
Sbjct: 969 TIMVNYN 975
>XP_005264612
          Length = 2242

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIMIDYN 9
           TIM++YN
Sbjct: 969 TIMVNYN 975
>NP_001207417
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TSTIMID 7
           T+TIMID
Sbjct: 395 TATIMID 401
>XP_006721183
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TSTIMID 7
           T+TIMID
Sbjct: 348 TATIMID 354
>NP_001248
          Length = 713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TSTIMID 7
           T+TIMID
Sbjct: 348 TATIMID 354
>NP_001207419
          Length = 459

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TSTIMID 7
           T+TIMID
Sbjct: 94  TATIMID 100
>NP_001207418
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TSTIMID 7
           T+TIMID
Sbjct: 309 TATIMID 315
>NP_006222
          Length = 2286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIMIDYN 9
           TIM+ YN
Sbjct: 357 TIMVTYN 363
>NP_003092
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   STIMIDY 8
           ST+++DY
Sbjct: 155 STLVVDY 161
>NP_001239441
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  STIMIDY 8
          ST+++DY
Sbjct: 90 STLVVDY 96
>NP_002079
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STIMIDYN 9
           STI+ID N
Sbjct: 212 STIIIDAN 219
>NP_001239440
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   STIMIDY 8
           ST+++DY
Sbjct: 97  STLVVDY 103
>NP_077015
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   TSTIMIDY 8
           T  +M+DY
Sbjct: 613 TQIVMVDY 620
>XP_006723233
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STIMIDYN 9
           STI+ID N
Sbjct: 144 STIIIDAN 151
>XP_006723232
          Length = 981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STIMIDYN 9
           STI+ID N
Sbjct: 212 STIIIDAN 219
>XP_005258879
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STIMIDYN 9
           STI+ID N
Sbjct: 212 STIIIDAN 219
>XP_005258878
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STIMIDYN 9
           STI+ID N
Sbjct: 212 STIIIDAN 219
>NP_001244899
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   TSTIMIDY 8
           T  +M+DY
Sbjct: 613 TQIVMVDY 620
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.128    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 368,639
Number of extensions: 554
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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