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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1135
NP_009297                                                              18    1135
NP_002070                                                              15    9605
NP_001353                                                              15    9605
NP_076413                                                              15    9605
XP_006714556                                                           15    9605
XP_006714555                                                           15    9605
NP_001009877                                                           15    9605

>NP_005148
          Length = 1130

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSRLAIGE 9
           TSSRLA GE
Sbjct: 694 TSSRLATGE 702



>NP_009297
          Length = 1149

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSRLAIGE 9
           TSSRLA GE
Sbjct: 713 TSSRLATGE 721



>NP_002070
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SSRLAIGE 9
          +SRLA+G+
Sbjct: 84 ASRLALGD 91



>NP_001353
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SSRLAIGE 9
          +SRL +GE
Sbjct: 6  TSRLVVGE 13



>NP_076413
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SRLAIGE 9
           SRL++GE
Sbjct: 568 SRLSVGE 574



>XP_006714556
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SRLAIGE 9
           SRL++GE
Sbjct: 588 SRLSVGE 594



>XP_006714555
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SRLAIGE 9
           SRL++GE
Sbjct: 567 SRLSVGE 573



>NP_001009877
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SRLAIGE 9
           SRL++GE
Sbjct: 515 SRLSVGE 521



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.120    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 258,796
Number of extensions: 203
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)