Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           20     299
NP_001257437                                                           20     299
NP_006281                                                              20     299
XP_006715618                                                           20     299
XP_005267176                                                           20     299
XP_005276276                                                           17    4312
XP_005263905                                                           17    4312
XP_005275986                                                           17    4312
NP_002372                                                              16    7355
NP_219494                                                              16    7355
NP_001156863                                                           16    7355
NP_002282                                                              16    7355
XP_006715347                                                           16    7355
XP_006715346                                                           16    7355
XP_006715345                                                           16    7355
NP_000937                                                              15    9605
NP_001185744                                                           15    9605
XP_005276293                                                           15    9605
XP_005275558                                                           15    9605
XP_005275391                                                           15    9605
XP_005275259                                                           15    9605
XP_005274965                                                           15    9605
XP_005272815                                                           15    9605
XP_005274885                                                           15    9605
XP_005248836                                                           15    9605
XP_005264833                                                           15    9605
XP_005276006                                                           15    9605
>NP_001257436
          Length = 790

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSSRFQNTI 9
           T+SRFQNTI
Sbjct: 647 TASRFQNTI 655
>NP_001257437
          Length = 790

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSSRFQNTI 9
           T+SRFQNTI
Sbjct: 647 TASRFQNTI 655
>NP_006281
          Length = 790

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSSRFQNTI 9
           T+SRFQNTI
Sbjct: 647 TASRFQNTI 655
>XP_006715618
          Length = 577

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSSRFQNTI 9
           T+SRFQNTI
Sbjct: 434 TASRFQNTI 442
>XP_005267176
          Length = 790

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSSRFQNTI 9
           T+SRFQNTI
Sbjct: 647 TASRFQNTI 655
>XP_005276276
          Length = 302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TSSRFQNT 8
          TSSR QNT
Sbjct: 13 TSSRNQNT 20
>XP_005263905
          Length = 302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TSSRFQNT 8
          TSSR QNT
Sbjct: 13 TSSRNQNT 20
>XP_005275986
          Length = 302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TSSRFQNT 8
          TSSR QNT
Sbjct: 13 TSSRNQNT 20
>NP_002372
          Length = 486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSRFQNT 8
           SSRFQ T
Sbjct: 255 SSRFQET 261
>NP_219494
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RFQNTI 9
           RFQNT+
Sbjct: 316 RFQNTL 321
>NP_001156863
          Length = 611

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TSSRFQN 7
           TSS+F+N
Sbjct: 209 TSSKFEN 215
>NP_002282
          Length = 1786

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TSSRFQNTI 9
           TSSR  NTI
Sbjct: 639 TSSRCGNTI 647
>XP_006715347
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TSSRFQN 7
           TSS+F+N
Sbjct: 209 TSSKFEN 215
>XP_006715346
          Length = 611

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TSSRFQN 7
           TSS+F+N
Sbjct: 209 TSSKFEN 215
>XP_006715345
          Length = 611

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TSSRFQN 7
           TSS+F+N
Sbjct: 209 TSSKFEN 215
>NP_000937
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSRFQNTI 9
           +SR+QN I
Sbjct: 276 ASRYQNNI 283
>NP_001185744
          Length = 1773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1625 TSSTFQET 1632
>XP_005276293
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRFQNT 8
           S+FQNT
Sbjct: 342 SKFQNT 347
>XP_005275558
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005275391
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005275259
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005274965
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005272815
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005274885
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005248836
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TSSRFQNT 8
            TSS FQ T
Sbjct: 1628 TSSTFQET 1635
>XP_005264833
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRFQNT 8
           S+FQNT
Sbjct: 342 SKFQNT 347
>XP_005276006
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRFQNT 8
           S+FQNT
Sbjct: 342 SKFQNT 347
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.116    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,657
Number of extensions: 385
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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