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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_055951                                                              16    7355
NP_065931                                                              16    7355
XP_006723257                                                           16    7355
XP_006723256                                                           16    7355
XP_006715929                                                           16    7355
XP_005250018                                                           16    7355
XP_005250017                                                           16    7355
XP_005250016                                                           16    7355
XP_006715928                                                           16    7355
XP_006715927                                                           16    7355
XP_006713129                                                           16    7355
XP_006713128                                                           16    7355
XP_005265032                                                           16    7355
XP_005265031                                                           16    7355
XP_005265030                                                           16    7355
NP_997219                                                              16    7355
NP_001177894                                                           15    9605
NP_001177893                                                           15    9605
NP_932072                                                              15    9605
NP_003444                                                              15    9605
NP_006769                                                              15    9605
XP_005260637                                                           15    9605
XP_005260636                                                           15    9605
XP_006722105                                                           15    9605
XP_005258083                                                           15    9605
XP_005258084                                                           15    9605
XP_006719927                                                           15    9605
XP_005266578                                                           15    9605
XP_005266576                                                           15    9605
XP_005266575                                                           15    9605
XP_005266577                                                           15    9605
XP_005266574                                                           15    9605
NP_003161                                                              15    9605

>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SSFSRKTM 9
            SSFSRKTM
Sbjct: 2124 SSFSRKTM 2131



>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SSFSRKTM 9
            SSFSRKTM
Sbjct: 1919 SSFSRKTM 1926



>NP_055951
          Length = 2570

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SSFSRKTM 9
           SSFS +TM
Sbjct: 405 SSFSSRTM 412



>NP_065931
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 275 SFSRKT 280



>XP_006723257
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 275 SFSRKT 280



>XP_006723256
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 275 SFSRKT 280



>XP_006715929
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_005250018
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_005250017
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_005250016
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_006715928
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 555 SFSRKT 560



>XP_006715927
          Length = 636

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 592 SFSRKT 597



>XP_006713129
          Length = 1521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SSFSRKTM 9
           SSFS +TM
Sbjct: 405 SSFSSRTM 412



>XP_006713128
          Length = 1619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SSFSRKTM 9
           SSFS +TM
Sbjct: 405 SSFSSRTM 412



>XP_005265032
          Length = 2545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SSFSRKTM 9
           SSFS +TM
Sbjct: 405 SSFSSRTM 412



>XP_005265031
          Length = 2569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SSFSRKTM 9
           SSFS +TM
Sbjct: 405 SSFSSRTM 412



>XP_005265030
          Length = 2595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SSFSRKTM 9
           SSFS +TM
Sbjct: 405 SSFSSRTM 412



>NP_997219
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRKT 8
           SFSRKT
Sbjct: 551 SFSRKT 556



>NP_001177894
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>NP_001177893
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>NP_932072
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>NP_003444
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>NP_006769
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSSFSRKTM 9
           T+SF+RK +
Sbjct: 457 TASFTRKVI 465



>XP_005260637
          Length = 132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSFSRK 7
          SSFSRK
Sbjct: 23 SSFSRK 28



>XP_005260636
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSFSRK 7
          SSFSRK
Sbjct: 23 SSFSRK 28



>XP_006722105
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSFSRK 7
           SSFSRK
Sbjct: 223 SSFSRK 228



>XP_005258083
          Length = 1726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSFSRK 7
           SSFSRK
Sbjct: 346 SSFSRK 351



>XP_005258084
          Length = 1726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSFSRK 7
           SSFSRK
Sbjct: 346 SSFSRK 351



>XP_006719927
          Length = 1159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>XP_005266578
          Length = 1290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>XP_005266576
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>XP_005266575
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>XP_005266577
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>XP_005266574
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSSFSR 6
           TSSFSR
Sbjct: 157 TSSFSR 162



>NP_003161
          Length = 1726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSFSRK 7
           SSFSRK
Sbjct: 346 SSFSRK 351



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.105    0.252 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,614
Number of extensions: 724
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)