Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     869
XP_005262424                                                           19     869
NP_036547                                                              15    9605
NP_001192192                                                           15    9605
NP_008879                                                              15    9605
NP_001099001                                                           15    9605
NP_065870                                                              15    9605
NP_001156001                                                           15    9605
XP_005248808                                                           15    9605
XP_005248806                                                           15    9605
XP_005248805                                                           15    9605
XP_005248802                                                           15    9605
XP_005248801                                                           15    9605
XP_005248800                                                           15    9605
>NP_722576
          Length = 3080

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TSQMVEFSV 9
            TSQMVEF V
Sbjct: 1857 TSQMVEFPV 1865
>XP_005262424
          Length = 2875

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TSQMVEFSV 9
            TSQMVEF V
Sbjct: 1652 TSQMVEFPV 1660
>NP_036547
          Length = 910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSQMVEFSV 9
           TS+ ++FSV
Sbjct: 798 TSEHIQFSV 806
>NP_001192192
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSQMVEFSV 9
           TS+ ++FSV
Sbjct: 614 TSEHIQFSV 622
>NP_008879
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MVEFSV 9
           MVEFSV
Sbjct: 129 MVEFSV 134
>NP_001099001
          Length = 1319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>NP_065870
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>NP_001156001
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248808
          Length = 1121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248806
          Length = 1317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248805
          Length = 1345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248802
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248801
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248800
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MVEFSV 9
          MVEFSV
Sbjct: 7  MVEFSV 12
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.116    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 322,647
Number of extensions: 411
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package