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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_598328                                                              18    1135
NP_008881                                                              18    1135
XP_006713376                                                           18    1135
XP_006713375                                                           18    1135
NP_001129246                                                           17    2528
NP_003481                                                              17    2528
NP_510880                                                              16    7355
NP_976063                                                              16    7355
NP_058649                                                              15    9605

>NP_598328
          Length = 582

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKSFAS 9
           SLRKSFAS
Sbjct: 571 SLRKSFAS 578



>NP_008881
          Length = 705

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKSFAS 9
           SLRKSFAS
Sbjct: 694 SLRKSFAS 701



>XP_006713376
          Length = 325

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKSFAS 9
           SLRKSFAS
Sbjct: 314 SLRKSFAS 321



>XP_006713375
          Length = 421

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKSFAS 9
           SLRKSFAS
Sbjct: 410 SLRKSFAS 417



>NP_001129246
          Length = 579

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SLRKSFAS 9
           +LRKSFAS
Sbjct: 568 NLRKSFAS 575



>NP_003481
          Length = 580

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SLRKSFAS 9
           +LRKSFAS
Sbjct: 569 NLRKSFAS 576



>NP_510880
          Length = 2054

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TSLRKSFAS 9
            TS+RK+F +
Sbjct: 1003 TSMRKTFTT 1011



>NP_976063
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TSLRKSFAS 9
            TS+RK+F +
Sbjct: 1003 TSMRKTFTT 1011



>NP_058649
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LRKSFA 8
           LRKSFA
Sbjct: 121 LRKSFA 126



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.112    0.263 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 393,610
Number of extensions: 428
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)