Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results 
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done




                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           19     869
NP_060785                                                              19     869
NP_361014                                                              19     869
NP_001073936                                                           18    1935
NP_001074319                                                           17    3301
NP_000250                                                              17    4312
NP_001135967                                                           17    4312
NP_001307                                                              17    4312
XP_005254459                                                           17    4312
XP_005254458                                                           17    4312
XP_005254457                                                           17    4312
XP_005254456                                                           17    4312
XP_005254455                                                           17    4312
XP_005254454                                                           17    4312
NP_005086                                                              16    5631
NP_001036083                                                           16    5631
NP_005475                                                              16    5631
XP_005267304                                                           16    5631
XP_005271388                                                           16    5631
XP_006711098                                                           16    5631
XP_006711097                                                           16    5631
XP_005271387                                                           16    5631
XP_005271385                                                           16    5631
NP_001257903                                                           16    7355
XP_006721269                                                           16    7355
NP_945314                                                              15    9605
NP_775871                                                              15    9605
NP_057279                                                              15    9605
NP_001271188                                                           15    9605



>NP_001013433
          Length = 589

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           TSGMELK N
Sbjct: 466 TSGMELKDN 474





>NP_060785
          Length = 627

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           TSGMELK N
Sbjct: 504 TSGMELKDN 512





>NP_361014
          Length = 707

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           TSGMELK N
Sbjct: 584 TSGMELKDN 592





>NP_001073936
          Length = 1848

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    TSGMELKYN 9
            ++GM+L+YN
Sbjct: 1707 STGMQLRYN 1715





>NP_001074319
          Length = 783

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           +SG +LKYN
Sbjct: 228 SSGEKLKYN 236





>NP_000250
          Length = 1855

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1714 SKGMQIRYN 1722





>NP_001135967
          Length = 1828

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1687 SKGMQIRYN 1695





>NP_001307
          Length = 971

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           T+G E+KY+
Sbjct: 303 TTGQEVKYD 311





>XP_005254459
          Length = 1825

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1684 SKGMQIRYN 1692





>XP_005254458
          Length = 1852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1711 SKGMQIRYN 1719





>XP_005254457
          Length = 1853

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1712 SKGMQIRYN 1720





>XP_005254456
          Length = 1877

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1736 SKGMQIRYN 1744





>XP_005254455
          Length = 1880

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1739 SKGMQIRYN 1747





>XP_005254454
          Length = 1880

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    TSGMELKYN 9
            + GM+++YN
Sbjct: 1739 SKGMQIRYN 1747





>NP_005086
          Length = 1548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SGMELKY 8
            SGM LKY
Sbjct: 1201 SGMTLKY 1207





>NP_001036083
          Length = 570

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MELKYN 9
           ME+KYN
Sbjct: 243 MEMKYN 248





>NP_005475
          Length = 583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MELKYN 9
           ME+KYN
Sbjct: 256 MEMKYN 261





>XP_005267304
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MELKYN 9
           ME+KYN
Sbjct: 256 MEMKYN 261





>XP_005271388
          Length = 1224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SGMELKY 8
           SGM LKY
Sbjct: 877 SGMTLKY 883





>XP_006711098
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SGMELKY 8
            SGM LKY
Sbjct: 1169 SGMTLKY 1175





>XP_006711097
          Length = 1542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SGMELKY 8
            SGM LKY
Sbjct: 1195 SGMTLKY 1201





>XP_005271387
          Length = 1545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SGMELKY 8
            SGM LKY
Sbjct: 1198 SGMTLKY 1204





>XP_005271385
          Length = 1551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SGMELKY 8
            SGM LKY
Sbjct: 1204 SGMTLKY 1210





>NP_001257903
          Length = 5121

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    TSGMELKY 8
            TSGME+ +
Sbjct: 4582 TSGMEVSF 4589





>XP_006721269
          Length = 5138

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    TSGMELKY 8
            TSGME+ +
Sbjct: 4599 TSGMEVSF 4606





>NP_945314
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TSGMELKY 8
           T G+EL+Y
Sbjct: 273 TIGLELEY 280





>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    SGMELKYN 9
            SG+  KYN
Sbjct: 1079 SGLVFKYN 1086





>NP_057279
          Length = 1780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           T+ +ELK+N
Sbjct: 670 TACLELKFN 678





>NP_001271188
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGMELKYN 9
           T+ +ELK+N
Sbjct: 710 TACLELKFN 718





  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.122    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 570,083
Number of extensions: 1001
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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