Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_659206                                                              17    2528
NP_659205                                                              17    2528
NP_006790                                                              17    2528
NP_001137139                                                           16    5631
NP_067034                                                              16    5631
XP_006718569                                                           16    5631
XP_005273977                                                           16    5631
XP_006718568                                                           16    5631
XP_005273975                                                           16    5631
XP_006718566                                                           16    5631
XP_006718565                                                           16    5631
NP_940867                                                              16    7355
NP_002652                                                              16    7355
NP_001243725                                                           16    7355
NP_054772                                                              16    7355
NP_001248326                                                           16    7355
NP_001248327                                                           16    7355
NP_002551                                                              16    7355
XP_006724697                                                           16    7355
XP_006724696                                                           16    7355
XP_006724695                                                           16    7355
XP_006724694                                                           16    7355
XP_006716820                                                           16    7355
XP_006716819                                                           16    7355
XP_006716818                                                           16    7355
NP_002347                                                              16    7355
NP_002267                                                              15    9605
NP_060037                                                              15    9605
NP_031370                                                              15    9605
NP_001192225                                                           15    9605
NP_438169                                                              15    9605
NP_001073159                                                           15    9605
NP_001273389                                                           15    9605
NP_001273388                                                           15    9605
NP_001273390                                                           15    9605
NP_001190173                                                           15    9605
NP_060363                                                              15    9605
XP_005254323                                                           15    9605
XP_005254322                                                           15    9605
XP_006719953                                                           15    9605
XP_005270067                                                           15    9605
XP_005270066                                                           15    9605
XP_005270065                                                           15    9605
XP_006717338                                                           15    9605
XP_006717337                                                           15    9605
XP_006717336                                                           15    9605
XP_006715560                                                           15    9605
XP_006712108                                                           15    9605
XP_005264472                                                           15    9605
XP_005264470                                                           15    9605
XP_005264469                                                           15    9605
>NP_659206
          Length = 300

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSFRK I
Sbjct: 210 KYSFRKDI 217
>NP_659205
          Length = 312

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSFRK I
Sbjct: 208 KYSFRKDI 215
>NP_006790
          Length = 314

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSFRK I
Sbjct: 210 KYSFRKDI 217
>NP_001137139
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RYSFRKSI 9
           RY F KSI
Sbjct: 294 RYRFNKSI 301
>NP_067034
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RYSFRKSI 9
           RY F KSI
Sbjct: 294 RYRFNKSI 301
>XP_006718569
          Length = 148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RYSFRKS 8
           RY FRKS
Sbjct: 139 RYFFRKS 145
>XP_005273977
          Length = 176

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RYSFRKS 8
           RY FRKS
Sbjct: 167 RYFFRKS 173
>XP_006718568
          Length = 215

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RYSFRKS 8
           RY FRKS
Sbjct: 206 RYFFRKS 212
>XP_005273975
          Length = 254

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RYSFRKS 8
           RY FRKS
Sbjct: 245 RYFFRKS 251
>XP_006718566
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RYSFRKS 8
           RY FRKS
Sbjct: 284 RYFFRKS 290
>XP_006718565
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RYSFRKS 8
           RY FRKS
Sbjct: 284 RYFFRKS 290
>NP_940867
          Length = 1582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSF K+I
Sbjct: 99  KYSFHKNI 106
>NP_002652
          Length = 1265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  TRYSFRKS 8
          T +SFRKS
Sbjct: 29 TVFSFRKS 36
>NP_001243725
          Length = 404

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RYSFRK 7
           RYSFR+
Sbjct: 289 RYSFRR 294
>NP_054772
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YSFRKSI 9
           YS++KSI
Sbjct: 319 YSYKKSI 325
>NP_001248326
          Length = 361

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RYSFRK 7
           RYSFR+
Sbjct: 246 RYSFRR 251
>NP_001248327
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RYSFRK 7
           RYSFR+
Sbjct: 273 RYSFRR 278
>NP_002551
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RYSFRK 7
           RYSFR+
Sbjct: 273 RYSFRR 278
>XP_006724697
          Length = 1526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSF K+I
Sbjct: 99  KYSFHKNI 106
>XP_006724696
          Length = 1534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSF K+I
Sbjct: 99  KYSFHKNI 106
>XP_006724695
          Length = 1574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSF K+I
Sbjct: 99  KYSFHKNI 106
>XP_006724694
          Length = 1582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RYSFRKSI 9
           +YSF K+I
Sbjct: 99  KYSFHKNI 106
>XP_006716820
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  RYSFRK 7
          RYSF+K
Sbjct: 83 RYSFKK 88
>XP_006716819
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  RYSFRK 7
          RYSF+K
Sbjct: 83 RYSFKK 88
>XP_006716818
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  RYSFRK 7
          RYSF+K
Sbjct: 83 RYSFKK 88
>NP_002347
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RYSFRKS 8
           R+SF+KS
Sbjct: 157 RFSFKKS 163
>NP_002267
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 TRYSFRKS 8
         T YS+R+S
Sbjct: 2 TSYSYRQS 9
>NP_060037
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TRYSFRKS 8
           +RY FRK+
Sbjct: 277 SRYIFRKA 284
>NP_031370
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RYSFRKSI 9
           RYSFR  I
Sbjct: 585 RYSFRLHI 592
>NP_001192225
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  RYSFRKSI 9
          RYSFR  I
Sbjct: 70 RYSFRLHI 77
>NP_438169
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RYSFRKSI 9
          R+SFRK +
Sbjct: 37 RHSFRKHL 44
>NP_001073159
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RYSFRKSI 9
          R+SFRK +
Sbjct: 37 RHSFRKHL 44
>NP_001273389
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RYSFRKSI 9
          R+SFRK +
Sbjct: 37 RHSFRKHL 44
>NP_001273388
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RYSFRKSI 9
          R+SFRK +
Sbjct: 37 RHSFRKHL 44
>NP_001273390
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RYSFRKSI 9
          R+SFRK +
Sbjct: 37 RHSFRKHL 44
>NP_001190173
          Length = 702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   TRYSFR 6
           TRY FR
Sbjct: 184 TRYEFR 189
>NP_060363
          Length = 843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   TRYSFR 6
           TRY FR
Sbjct: 184 TRYEFR 189
>XP_005254323
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   RYSFRK 7
           RY FRK
Sbjct: 389 RYEFRK 394
>XP_005254322
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   RYSFRK 7
           RY FRK
Sbjct: 392 RYEFRK 397
>XP_006719953
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RYSFRKSI 9
          R+SFRK +
Sbjct: 37 RHSFRKHL 44
>XP_005270067
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   TRYSFR 6
           TRY FR
Sbjct: 184 TRYEFR 189
>XP_005270066
          Length = 843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   TRYSFR 6
           TRY FR
Sbjct: 184 TRYEFR 189
>XP_005270065
          Length = 843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   TRYSFR 6
           TRY FR
Sbjct: 184 TRYEFR 189
>XP_006717338
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RYSFRKSI 9
           RYSFR  I
Sbjct: 548 RYSFRLHI 555
>XP_006717337
          Length = 876

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RYSFRKSI 9
           RYSFR  I
Sbjct: 556 RYSFRLHI 563
>XP_006717336
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RYSFRKSI 9
           RYSFR  I
Sbjct: 612 RYSFRLHI 619
>XP_006715560
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   RYSFRK 7
           RY FRK
Sbjct: 159 RYKFRK 164
>XP_006712108
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TRYSFRKS 8
          +RY FRK+
Sbjct: 83 SRYIFRKA 90
>XP_005264472
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TRYSFRKS 8
           +RY FRK+
Sbjct: 180 SRYIFRKA 187
>XP_005264470
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TRYSFRKS 8
           +RY FRK+
Sbjct: 180 SRYIFRKA 187
>XP_005264469
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TRYSFRKS 8
           +RY FRK+
Sbjct: 180 SRYIFRKA 187
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.134    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 487,249
Number of extensions: 922
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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