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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001119588                                                           18    1135
NP_001119587                                                           18    1135
NP_001119589                                                           18    1135
NP_003171                                                              17    3301
XP_006723405                                                           17    3301
XP_006723404                                                           17    3301
XP_006723403                                                           17    3301
XP_006723402                                                           17    3301
XP_006723401                                                           17    3301
NP_085915                                                              16    7355
NP_001155220                                                           16    7355
NP_001244202                                                           15    9605
NP_001257382                                                           15    9605
NP_008945                                                              15    9605
NP_001257383                                                           15    9605
NP_955456                                                              15    9605
XP_005255835                                                           15    9605

>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVLAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119588
          Length = 209

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TRVLAMAIY 9
          TRV AMAIY
Sbjct: 23 TRVRAMAIY 31



>NP_001119587
          Length = 261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TRVLAMAIY 9
          TRV AMAIY
Sbjct: 23 TRVRAMAIY 31



>NP_001119589
          Length = 214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TRVLAMAIY 9
          TRV AMAIY
Sbjct: 23 TRVRAMAIY 31



>NP_003171
          Length = 386

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           RVL MA+Y
Sbjct: 189 RVLVMAVY 196



>XP_006723405
          Length = 382

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           RVL MA+Y
Sbjct: 186 RVLVMAVY 193



>XP_006723404
          Length = 383

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           RVL MA+Y
Sbjct: 186 RVLVMAVY 193



>XP_006723403
          Length = 385

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           RVL MA+Y
Sbjct: 189 RVLVMAVY 196



>XP_006723402
          Length = 386

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           RVL MA+Y
Sbjct: 189 RVLVMAVY 196



>XP_006723401
          Length = 386

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           RVL MA+Y
Sbjct: 189 RVLVMAVY 196



>NP_085915
          Length = 215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLAMAIY 9
           VLA+AIY
Sbjct: 155 VLALAIY 161



>NP_001155220
          Length = 173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLAMAIY 9
           VLA+AIY
Sbjct: 113 VLALAIY 119



>NP_001244202
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RVLAMAI 8
           RV+AMAI
Sbjct: 447 RVMAMAI 453



>NP_001257382
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           R +AMA+Y
Sbjct: 494 RFIAMALY 501



>NP_008945
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           R +AMA+Y
Sbjct: 610 RFIAMALY 617



>NP_001257383
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           R +AMA+Y
Sbjct: 610 RFIAMALY 617



>NP_955456
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           R +AMA+Y
Sbjct: 171 RFIAMALY 178



>XP_005255835
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVLAMAIY 9
           R +AMA+Y
Sbjct: 610 RFIAMALY 617



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.140    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 181,323
Number of extensions: 173
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)