Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              20     509
XP_006715292                                                           20     509
NP_006368                                                              16    5631
NP_005472                                                              16    5631
NP_060177                                                              16    5631
XP_006722663                                                           16    5631
XP_006716779                                                           16    5631
XP_005245991                                                           16    5631
NP_001137250                                                           16    5631
NP_001193453                                                           15    9605
NP_001193471                                                           15    9605
NP_116756                                                              15    9605
NP_116758                                                              15    9605
NP_001221                                                              15    9605
NP_116759                                                              15    9605
XP_005246968                                                           15    9605
XP_005246967                                                           15    9605
XP_005246966                                                           15    9605
XP_005246964                                                           15    9605
>NP_115927
          Length = 1321

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TRQTAAMF 8
           TRQTAAMF
Sbjct: 331 TRQTAAMF 338
>XP_006715292
          Length = 759

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TRQTAAMF 8
           TRQTAAMF
Sbjct: 318 TRQTAAMF 325
>NP_006368
          Length = 1591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    QTAAMFR 9
            QTA MFR
Sbjct: 1453 QTAGMFR 1459
>NP_005472
          Length = 877

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRQTAAM 7
           TRQTAA+
Sbjct: 512 TRQTAAL 518
>NP_060177
          Length = 903

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRQTAAMFR 9
           TR+ AA+F+
Sbjct: 106 TREVAALFK 114
>XP_006722663
          Length = 877

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRQTAAM 7
           TRQTAA+
Sbjct: 512 TRQTAAL 518
>XP_006716779
          Length = 2399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    QTAAMFR 9
            QTA MFR
Sbjct: 2261 QTAGMFR 2267
>XP_005245991
          Length = 905

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRQTAAMFR 9
           TR+ AA+F+
Sbjct: 106 TREVAALFK 114
>NP_001137250
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRQTAAMFR 9
           TR+ AA+F+
Sbjct: 106 TREVAALFK 114
>NP_001193453
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>NP_001193471
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>NP_116756
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>NP_116758
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>NP_001221
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>NP_116759
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>XP_005246968
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>XP_005246967
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>XP_005246966
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
>XP_005246964
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TRQTAAMFR 9
           TRQ  ++FR
Sbjct: 108 TRQRVSLFR 116
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.124    0.334 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 214,374
Number of extensions: 152
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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