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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     299
XP_005262424                                                           20     299
NP_004091                                                              17    4312
NP_742103                                                              17    4312
NP_742101                                                              17    4312
XP_006715436                                                           17    4312
XP_005266910                                                           17    4312
XP_005266909                                                           17    4312
XP_005266908                                                           17    4312
XP_005266907                                                           17    4312
NP_078966                                                              16    5631
NP_001108603                                                           16    5631
NP_000056                                                              16    5631
NP_065849                                                              16    5631
XP_006723004                                                           16    5631
XP_006720114                                                           16    5631
XP_006714560                                                           16    5631
XP_005248414                                                           16    5631
XP_005248413                                                           16    5631
XP_006714559                                                           16    5631
NP_001001872                                                           16    5631
NP_694984                                                              16    7355
XP_006724701                                                           16    7355
XP_005262172                                                           16    7355
XP_005262170                                                           16    7355
NP_001289                                                              15    9605
NP_065976                                                              15    9605
NP_817123                                                              15    9605
XP_006710856                                                           15    9605

>NP_722576
          Length = 3080

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    TRMTSNNTQ 9
            TRMTS+NTQ
Sbjct: 1868 TRMTSSNTQ 1876



>XP_005262424
          Length = 2875

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    TRMTSNNTQ 9
            TRMTS+NTQ
Sbjct: 1663 TRMTSSNTQ 1671



>NP_004091
          Length = 639

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 294 MTSNNT 299



>NP_742103
          Length = 639

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 294 MTSNNT 299



>NP_742101
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 271 MTSNNT 276



>XP_006715436
          Length = 585

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 240 MTSNNT 245



>XP_005266910
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 271 MTSNNT 276



>XP_005266909
          Length = 620

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 294 MTSNNT 299



>XP_005266908
          Length = 645

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 294 MTSNNT 299



>XP_005266907
          Length = 645

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSNNT 8
           MTSNNT
Sbjct: 294 MTSNNT 299



>NP_078966
          Length = 14507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2     RMTSNNTQ 9
             RMT N TQ
Sbjct: 14413 RMTRNGTQ 14420



>NP_001108603
          Length = 934

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           TR++SN+T+
Sbjct: 850 TRLSSNSTK 858



>NP_000056
          Length = 934

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           TR++SN+T+
Sbjct: 850 TRLSSNSTK 858



>NP_065849
          Length = 1018

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSNNTQ 9
           R+T NN+Q
Sbjct: 471 RLTENNSQ 478



>XP_006723004
          Length = 6443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    RMTSNNTQ 9
            RMT N TQ
Sbjct: 6349 RMTRNGTQ 6356



>XP_006720114
          Length = 670

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           ++MT++NTQ
Sbjct: 258 SQMTADNTQ 266



>XP_006714560
          Length = 814

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           TR++SN+T+
Sbjct: 730 TRLSSNSTK 738



>XP_005248414
          Length = 943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           TR++SN+T+
Sbjct: 859 TRLSSNSTK 867



>XP_005248413
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           TR++SN+T+
Sbjct: 869 TRLSSNSTK 877



>XP_006714559
          Length = 962

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           TR++SN+T+
Sbjct: 878 TRLSSNSTK 886



>NP_001001872
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   TRMTSNNTQ 9
           ++MT++NTQ
Sbjct: 258 SQMTADNTQ 266



>NP_694984
          Length = 1802

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TRMTSNN 7
           T+MT NN
Sbjct: 407 TKMTGNN 413



>XP_006724701
          Length = 1398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1 TRMTSNN 7
         T+MT NN
Sbjct: 3 TKMTGNN 9



>XP_005262172
          Length = 1631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TRMTSNN 7
           T+MT NN
Sbjct: 236 TKMTGNN 242



>XP_005262170
          Length = 1752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TRMTSNN 7
           T+MT NN
Sbjct: 407 TKMTGNN 413



>NP_001289
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TSNNTQ 9
           TSNNT+
Sbjct: 143 TSNNTE 148



>NP_065976
          Length = 1223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSNNTQ 9
           R T+NNT+
Sbjct: 274 RSTNNNTK 281



>NP_817123
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTSNNTQ 9
           MT++NTQ
Sbjct: 700 MTASNTQ 706



>XP_006710856
          Length = 978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RMTSNNTQ 9
          R T+NNT+
Sbjct: 29 RSTNNNTK 36



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295   0.0969    0.239 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 480,946
Number of extensions: 594
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)