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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_002435                                                              17    3301
XP_005262326                                                           17    3301
NP_001003716                                                           16    5631
NP_004250                                                              16    5631
NP_001003715                                                           16    5631
XP_005257880                                                           16    5631
XP_005257875                                                           16    5631
NP_065905                                                              16    7355
XP_006722578                                                           16    7355
XP_005266789                                                           16    7355
XP_006722577                                                           16    7355
XP_006722576                                                           16    7355
XP_005266788                                                           16    7355
XP_005266787                                                           16    7355
NP_776248                                                              15    9605
NP_065885                                                              15    9605
NP_001130038                                                           15    9605
NP_055986                                                              15    9605
NP_001257869                                                           15    9605
XP_006723668                                                           15    9605
XP_006723667                                                           15    9605
XP_006723666                                                           15    9605
XP_006723665                                                           15    9605
XP_005255257                                                           15    9605
XP_005255256                                                           15    9605
XP_005255255                                                           15    9605
XP_005255254                                                           15    9605
XP_005255252                                                           15    9605

>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    REQLAVEL 9
            REQLAVEL
Sbjct: 1910 REQLAVEL 1917



>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    REQLAVEL 9
            REQLAVEL
Sbjct: 1910 REQLAVEL 1917



>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    REQLAVEL 9
            REQLAVEL
Sbjct: 1863 REQLAVEL 1870



>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    REQLAVEL 9
            REQLAVEL
Sbjct: 1942 REQLAVEL 1949



>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    REQLAVEL 9
            REQLAVEL
Sbjct: 1942 REQLAVEL 1949



>NP_002435
          Length = 577

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   TREQLAVEL 9
           T+EQLA+E+
Sbjct: 413 TQEQLALEM 421



>XP_005262326
          Length = 578

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   TREQLAVEL 9
           T+EQLA+E+
Sbjct: 414 TQEQLALEM 422



>NP_001003716
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EQLAVEL 9
           EQLA+EL
Sbjct: 273 EQLAIEL 279



>NP_004250
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EQLAVEL 9
           EQLA+EL
Sbjct: 273 EQLAIEL 279



>NP_001003715
          Length = 435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EQLAVEL 9
           EQLA+EL
Sbjct: 273 EQLAIEL 279



>XP_005257880
          Length = 549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EQLAVEL 9
           EQLA+EL
Sbjct: 273 EQLAIEL 279



>XP_005257875
          Length = 1012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EQLAVEL 9
           EQLA+EL
Sbjct: 273 EQLAIEL 279



>NP_065905
          Length = 1216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 803 SREQLAVLL 811



>XP_006722578
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 447 SREQLAVLL 455



>XP_005266789
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 803 SREQLAVLL 811



>XP_006722577
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 803 SREQLAVLL 811



>XP_006722576
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 803 SREQLAVLL 811



>XP_005266788
          Length = 1192

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 779 SREQLAVLL 787



>XP_005266787
          Length = 1216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TREQLAVEL 9
           +REQLAV L
Sbjct: 803 SREQLAVLL 811



>NP_776248
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 189 TREQLA 194



>NP_065885
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TREQLAV 7
           T+EQLA+
Sbjct: 364 TKEQLAI 370



>NP_001130038
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   REQLAVEL 9
           ++QLA+EL
Sbjct: 529 KKQLAIEL 536



>NP_055986
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 870 TREQLA 875



>NP_001257869
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 856 TREQLA 861



>XP_006723668
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   REQLAVEL 9
           ++QLA+EL
Sbjct: 529 KKQLAIEL 536



>XP_006723667
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   REQLAVEL 9
           ++QLA+EL
Sbjct: 536 KKQLAIEL 543



>XP_006723666
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   REQLAVEL 9
           ++QLA+EL
Sbjct: 537 KKQLAIEL 544



>XP_006723665
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   REQLAVEL 9
           ++QLA+EL
Sbjct: 537 KKQLAIEL 544



>XP_005255257
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 519 TREQLA 524



>XP_005255256
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 525 TREQLA 530



>XP_005255255
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 696 TREQLA 701



>XP_005255254
          Length = 1051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 796 TREQLA 801



>XP_005255252
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TREQLA 6
           TREQLA
Sbjct: 869 TREQLA 874



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.126    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,066
Number of extensions: 497
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)