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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001119588                                                           18    1135
NP_001119587                                                           18    1135
NP_001119589                                                           18    1135
NP_000862                                                              16    5631
NP_078889                                                              16    7355
NP_005442                                                              15    9605
NP_976227                                                              15    9605
NP_067045                                                              15    9605
NP_001274324                                                           15    9605
XP_006715003                                                           15    9605
XP_006715002                                                           15    9605
XP_006715001                                                           15    9605
XP_006715000                                                           15    9605
XP_006712547                                                           15    9605
XP_005245454                                                           15    9605
XP_005245453                                                           15    9605
XP_005245450                                                           15    9605

>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 155 TRVRAMAIY 163



>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TPVRAMAIY 9
           T VRAMAIY
Sbjct: 116 TRVRAMAIY 124



>NP_001119588
          Length = 209

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TPVRAMAIY 9
          T VRAMAIY
Sbjct: 23 TRVRAMAIY 31



>NP_001119587
          Length = 261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TPVRAMAIY 9
          T VRAMAIY
Sbjct: 23 TRVRAMAIY 31



>NP_001119589
          Length = 214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TPVRAMAIY 9
          T VRAMAIY
Sbjct: 23 TRVRAMAIY 31



>NP_000862
          Length = 440

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TPVRAMAI 8
           TP+RA+A+
Sbjct: 139 TPLRALAL 146



>NP_078889
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   TPVRAMAIY 9
           TP ++ A+Y
Sbjct: 104 TPTKSFAVY 112



>NP_005442
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TPVRAMAIY 9
           TP+R  A Y
Sbjct: 372 TPIRNRAFY 380



>NP_976227
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TPVRAMAIY 9
           TP+R  A Y
Sbjct: 338 TPIRNRAFY 346



>NP_067045
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PVRAMAIY 9
           PVR +AI+
Sbjct: 295 PVRQLAIF 302



>NP_001274324
          Length = 1688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TPVRAMAI 8
           +PVRA+A+
Sbjct: 873 SPVRALAM 880



>XP_006715003
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TPVRAMAIY 9
           TP+R  A Y
Sbjct: 289 TPIRNRAFY 297



>XP_006715002
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TPVRAMAIY 9
           TP+R  A Y
Sbjct: 344 TPIRNRAFY 352



>XP_006715001
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TPVRAMAIY 9
           TP+R  A Y
Sbjct: 403 TPIRNRAFY 411



>XP_006715000
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TPVRAMAIY 9
           TP+R  A Y
Sbjct: 437 TPIRNRAFY 445



>XP_006712547
          Length = 1674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TPVRAMAI 8
           +PVRA+A+
Sbjct: 870 SPVRALAM 877



>XP_005245454
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PVRAMAIY 9
           PVR +AI+
Sbjct: 113 PVRQLAIF 120



>XP_005245453
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PVRAMAIY 9
           PVR +AI+
Sbjct: 113 PVRQLAIF 120



>XP_005245450
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PVRAMAIY 9
           PVR +AI+
Sbjct: 265 PVRQLAIF 272



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 348,085
Number of extensions: 381
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)