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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_001135419                                                           16    5631
NP_001012524                                                           16    5631
XP_005265144                                                           16    5631
XP_005265142                                                           16    5631
XP_005265141                                                           16    5631
XP_005265140                                                           16    5631
XP_006713178                                                           16    5631
XP_005265139                                                           16    5631
XP_005265138                                                           16    5631
NP_055524                                                              16    7355
NP_443165                                                              16    7355
NP_078808                                                              15    9605
NP_001020607                                                           15    9605
NP_060264                                                              15    9605
NP_000383                                                              15    9605
NP_690043                                                              15    9605
NP_000780                                                              15    9605
NP_001171528                                                           15    9605
NP_061938                                                              15    9605
XP_006721800                                                           15    9605
XP_005257167                                                           15    9605
XP_006721263                                                           15    9605
XP_006717693                                                           15    9605
XP_005251551                                                           15    9605
XP_006716857                                                           15    9605
XP_006712810                                                           15    9605
XP_006712809                                                           15    9605
XP_006712808                                                           15    9605

>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    TNFLLKMTS 9
            TNFLL+MTS
Sbjct: 2559 TNFLLEMTS 2567



>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    TNFLLKMTS 9
            TNFLL+MTS
Sbjct: 2559 TNFLLEMTS 2567



>NP_001135419
          Length = 948

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 439 TNFLRSMTA 447



>NP_001012524
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 405 TNFLRSMTA 413



>XP_005265144
          Length = 531

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 439 TNFLRSMTA 447



>XP_005265142
          Length = 590

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TNFLLKMTS 9
          TNFL  MT+
Sbjct: 75 TNFLRSMTA 83



>XP_005265141
          Length = 655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 140 TNFLRSMTA 148



>XP_005265140
          Length = 911

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 396 TNFLRSMTA 404



>XP_006713178
          Length = 944

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 429 TNFLRSMTA 437



>XP_005265139
          Length = 947

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 432 TNFLRSMTA 440



>XP_005265138
          Length = 954

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           TNFL  MT+
Sbjct: 439 TNFLRSMTA 447



>NP_055524
          Length = 3546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    TNFLLKMTS 9
            T+FLLK+ S
Sbjct: 3238 THFLLKVQS 3246



>NP_443165
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TNFLLKMT 8
          T+F+LK+T
Sbjct: 81 TSFMLKVT 88



>NP_078808
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 115 NFLIKM 120



>NP_001020607
          Length = 183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 115 NFLIKM 120



>NP_060264
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NF+LKM
Sbjct: 815 NFILKM 820



>NP_000383
          Length = 1545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NFLLKMTS 9
            N+L++MTS
Sbjct: 1253 NWLVRMTS 1260



>NP_690043
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 476 NFLMKM 481



>NP_000780
          Length = 1306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    NFLLKM 7
            NFL+KM
Sbjct: 1050 NFLMKM 1055



>NP_001171528
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 476 NFLMKM 481



>NP_061938
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNFLLKMTS 9
           T FLLK T+
Sbjct: 166 TRFLLKETA 174



>XP_006721800
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 360 NFLMKM 365



>XP_005257167
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 867 NFLMKM 872



>XP_006721263
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TNFLLKMTS 9
          T FLLK T+
Sbjct: 40 TRFLLKETA 48



>XP_006717693
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NFLLKMTS 9
            N+L++MTS
Sbjct: 1021 NWLVRMTS 1028



>XP_005251551
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NF+LKM
Sbjct: 815 NFILKM 820



>XP_006716857
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NF+LKM
Sbjct: 848 NFILKM 853



>XP_006712810
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 115 NFLIKM 120



>XP_006712809
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 115 NFLIKM 120



>XP_006712808
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFLLKM 7
           NFL+KM
Sbjct: 115 NFLIKM 120



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.127    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 421,907
Number of extensions: 742
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)