Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_001027006                                                           17    2528
NP_001243665                                                           17    4312
NP_036578                                                              17    4312
NP_001243663                                                           17    4312
NP_001243664                                                           17    4312
NP_001243662                                                           17    4312
NP_001243661                                                           17    4312
XP_006726443                                                           17    4312
XP_005264766                                                           17    4312
NP_073600                                                              16    5631
NP_001128567                                                           16    5631
NP_612138                                                              16    5631
XP_006719828                                                           16    5631
NP_001177255                                                           16    7355
NP_073557                                                              16    7355
NP_006173                                                              16    7355
NP_001014796                                                           16    7355
NP_000048                                                              16    7355
NP_001274177                                                           16    7355
NP_001136093                                                           16    7355
NP_004248                                                              16    7355
NP_001274176                                                           16    7355
NP_001274175                                                           16    7355
NP_001264198                                                           16    7355
NP_699179                                                              16    7355
XP_005253925                                                           16    7355
XP_005253924                                                           16    7355
XP_006712924                                                           16    7355
XP_006712923                                                           16    7355
XP_006711408                                                           16    7355
XP_006711407                                                           16    7355
XP_005245278                                                           16    7355
NP_036306                                                              16    7355
NP_001494                                                              15    9605
NP_001242                                                              15    9605
NP_001035148                                                           15    9605
NP_056049                                                              15    9605
NP_001091988                                                           15    9605
NP_001264214                                                           15    9605
NP_002548                                                              15    9605
NP_002510                                                              15    9605
NP_849144                                                              15    9605
XP_006724995                                                           15    9605
XP_006720676                                                           15    9605
XP_005254608                                                           15    9605
XP_006718858                                                           15    9605
XP_005249075                                                           15    9605
XP_005248952                                                           15    9605
XP_005248951                                                           15    9605
XP_005248950                                                           15    9605
XP_005248949                                                           15    9605
XP_006713662                                                           15    9605
XP_006713661                                                           15    9605
XP_006713660                                                           15    9605
XP_005247434                                                           15    9605
XP_006713659                                                           15    9605
XP_005273174                                                           15    9605
NP_001005522                                                           15    9605
NP_056307                                                              15    9605
NP_001004692                                                           15    9605
NP_001004695                                                           15    9605
>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TNALSFPYT 9
            T ALSFPYT
Sbjct: 1182 TYALSFPYT 1190
>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TNALSFPYT 9
           T ALSFPYT
Sbjct: 977 TYALSFPYT 985
>NP_001027006
          Length = 1149

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    TNALSFP 7
            TN+LSFP
Sbjct: 1096 TNSLSFP 1102
>NP_001243665
          Length = 298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           TNA S PY+
Sbjct: 195 TNANSIPYS 203
>NP_036578
          Length = 361

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           TNA S PY+
Sbjct: 225 TNANSIPYS 233
>NP_001243663
          Length = 339

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           TNA S PY+
Sbjct: 203 TNANSIPYS 211
>NP_001243664
          Length = 331

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           TNA S PY+
Sbjct: 195 TNANSIPYS 203
>NP_001243662
          Length = 341

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           TNA S PY+
Sbjct: 205 TNANSIPYS 213
>NP_001243661
          Length = 369

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           TNA S PY+
Sbjct: 233 TNANSIPYS 241
>XP_006726443
          Length = 258

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           T  L+FPYT
Sbjct: 208 TCELTFPYT 216

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           T  L+FPYT
Sbjct: 224 TCELTFPYT 232

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           T  L+FPYT
Sbjct: 232 TCELTFPYT 240
>XP_005264766
          Length = 290

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           T  L+FPYT
Sbjct: 240 TCELTFPYT 248

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           T  L+FPYT
Sbjct: 256 TCELTFPYT 264

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TNALSFPYT 9
           T  L+FPYT
Sbjct: 264 TCELTFPYT 272
>NP_073600
          Length = 1204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NALSFPYT 9
           + LSFPY+
Sbjct: 308 SGLSFPYS 315
>NP_001128567
          Length = 1204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NALSFPYT 9
           + LSFPY+
Sbjct: 308 SGLSFPYS 315
>NP_612138
          Length = 240

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  LSFPYT 9
          LS+PYT
Sbjct: 55 LSYPYT 60
>XP_006719828
          Length = 640

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TNALSFPYT 9
           T +L+FPY+
Sbjct: 266 TASLAFPYS 274
>NP_001177255
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           AL+FPY
Sbjct: 234 ALNFPY 239
>NP_073557
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           AL+FPY
Sbjct: 234 ALNFPY 239
>NP_006173
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NALSFP 7
           NA+SFP
Sbjct: 316 NAISFP 321
>NP_001014796
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NALSFP 7
           NA+SFP
Sbjct: 316 NAISFP 321
>NP_000048
          Length = 1417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALSFPYT 9
           +LSFP+T
Sbjct: 646 SLSFPHT 652
>NP_001274177
          Length = 1042

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALSFPYT 9
           +LSFP+T
Sbjct: 271 SLSFPHT 277
>NP_001136093
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           A+SFPY
Sbjct: 254 AVSFPY 259
>NP_004248
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           A+SFPY
Sbjct: 254 AVSFPY 259
>NP_001274176
          Length = 1286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALSFPYT 9
           +LSFP+T
Sbjct: 646 SLSFPHT 652
>NP_001274175
          Length = 1417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALSFPYT 9
           +LSFP+T
Sbjct: 646 SLSFPHT 652
>NP_001264198
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    TNALSFPYT 9
            TNA   PYT
Sbjct: 2905 TNATWVPYT 2913
>NP_699179
          Length = 598

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           AL+FPY
Sbjct: 590 ALAFPY 595
>XP_005253925
          Length = 417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           AL+FPY
Sbjct: 409 ALAFPY 414
>XP_005253924
          Length = 417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           AL+FPY
Sbjct: 409 ALAFPY 414
>XP_006712924
          Length = 819

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           A+SFPY
Sbjct: 254 AVSFPY 259
>XP_006712923
          Length = 824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           A+SFPY
Sbjct: 254 AVSFPY 259
>XP_006711408
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NALSFP 7
           NA+SFP
Sbjct: 328 NAISFP 333
>XP_006711407
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NALSFP 7
           NA+SFP
Sbjct: 316 NAISFP 321
>XP_005245278
          Length = 867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NALSFP 7
           NA+SFP
Sbjct: 328 NAISFP 333
>NP_036306
          Length = 532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALSFPY 8
           AL+FPY
Sbjct: 524 ALAFPY 529
>NP_001494
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 374 LSFPY 378
>NP_001242
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 216 LSFPY 220
>NP_001035148
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 189 LSFPY 193
>NP_056049
          Length = 1346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NALSFP 7
          N+LSFP
Sbjct: 85 NSLSFP 90
>NP_001091988
          Length = 1346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NALSFP 7
          N+LSFP
Sbjct: 85 NSLSFP 90
>NP_001264214
          Length = 1437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NALSFPYT 9
           N L+FP T
Sbjct: 600 NTLAFPIT 607
>NP_002548
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NALSFPY 8
           NA+SF Y
Sbjct: 333 NAISFSY 339
>NP_002510
          Length = 1427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    NALSFP 7
            NA+SFP
Sbjct: 1091 NAVSFP 1096
>NP_849144
          Length = 2623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    TNALSFP 7
            T ALSFP
Sbjct: 1076 TAALSFP 1082
>XP_006724995
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NALSFPY 8
           NA+S PY
Sbjct: 430 NAISSPY 436
>XP_006720676
          Length = 938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 376 LSFPY 380
>XP_005254608
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 376 LSFPY 380
>XP_006718858
          Length = 913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  NALSFPYT 9
          N L+FP T
Sbjct: 76 NTLAFPIT 83
>XP_005249075
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 211 LSFPY 215
>XP_005248952
          Length = 1380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NALSFP 7
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_005248951
          Length = 1381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NALSFP 7
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_005248950
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NALSFP 7
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_005248949
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NALSFP 7
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_006713662
          Length = 1846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    TNALSFP 7
            T ALSFP
Sbjct: 1148 TAALSFP 1154
>XP_006713661
          Length = 1860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    TNALSFP 7
            T ALSFP
Sbjct: 1148 TAALSFP 1154
>XP_006713660
          Length = 2623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    TNALSFP 7
            T ALSFP
Sbjct: 1076 TAALSFP 1082
>XP_005247434
          Length = 2623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    TNALSFP 7
            T ALSFP
Sbjct: 1076 TAALSFP 1082
>XP_006713659
          Length = 2695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    TNALSFP 7
            T ALSFP
Sbjct: 1148 TAALSFP 1154
>XP_005273174
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 162 LSFPY 166
>NP_001005522
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 162 LSFPY 166
>NP_056307
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 376 LSFPY 380
>NP_001004692
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 162 LSFPY 166
>NP_001004695
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   LSFPY 8
           LSFPY
Sbjct: 162 LSFPY 166
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.126    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 643,896
Number of extensions: 627
Number of successful extensions: 67
Number of sequences better than 10000.0: 63
Number of HSP's gapped: 67
Number of HSP's successfully gapped: 67
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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