Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     299
NP_000170                                                              20     299
NP_001268422                                                           20     299
NP_001268423                                                           20     299
XP_005264328                                                           20     299
NP_001269470                                                           18    1135
NP_003167                                                              18    1135
NP_001269471                                                           18    1135
XP_005271213                                                           18    1135
XP_005271212                                                           18    1135
XP_005271211                                                           18    1135
XP_006710922                                                           18    1135
NP_065385                                                              17    3301
NP_055568                                                              16    7355
NP_055959                                                              16    7355
NP_689450                                                              16    7355
XP_006726562                                                           16    7355
XP_005276411                                                           16    7355
XP_005276410                                                           16    7355
XP_006726561                                                           16    7355
XP_005276409                                                           16    7355
XP_005276408                                                           16    7355
XP_005276407                                                           16    7355
XP_006726559                                                           16    7355
XP_005276406                                                           16    7355
XP_006726558                                                           16    7355
XP_006724974                                                           16    7355
XP_005257319                                                           16    7355
XP_005257318                                                           16    7355
XP_005257317                                                           16    7355
XP_005255915                                                           16    7355
XP_005271906                                                           16    7355
XP_006718144                                                           16    7355
XP_005271905                                                           16    7355
XP_005271904                                                           16    7355
XP_005271903                                                           16    7355
XP_006716964                                                           16    7355
XP_006716963                                                           16    7355
XP_006716962                                                           16    7355
XP_006716961                                                           16    7355
NP_037455                                                              16    7355
NP_037473                                                              15    9605
NP_653081                                                              15    9605
NP_055650                                                              15    9605
NP_002431                                                              15    9605
NP_001138590                                                           15    9605
XP_005257785                                                           15    9605
XP_006718300                                                           15    9605
XP_006718299                                                           15    9605
XP_005252990                                                           15    9605
NP_004305                                                              15    9605
NP_006811                                                              15    9605
NP_115510                                                              15    9605
>NP_001268421
          Length = 1230

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MSGESTFF 9
            MSGESTFF
Sbjct: 1054 MSGESTFF 1061
>NP_000170
          Length = 1360

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MSGESTFF 9
            MSGESTFF
Sbjct: 1184 MSGESTFF 1191
>NP_001268422
          Length = 1058

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MSGESTFF 9
           MSGESTFF
Sbjct: 882 MSGESTFF 889
>NP_001268423
          Length = 1058

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MSGESTFF 9
           MSGESTFF
Sbjct: 882 MSGESTFF 889
>XP_005264328
          Length = 1261

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MSGESTFF 9
            MSGESTFF
Sbjct: 1085 MSGESTFF 1092
>NP_001269470
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>NP_003167
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>NP_001269471
          Length = 951

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>XP_005271213
          Length = 921

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>XP_005271212
          Length = 958

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>XP_005271211
          Length = 973

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>XP_006710922
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TMSGESTFF 9
          T+ G+STFF
Sbjct: 27 TLGGDSTFF 35
>NP_065385
          Length = 731

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  TMSGESTFF 9
          TM GE T F
Sbjct: 55 TMDGEGTLF 63
>NP_055568
          Length = 1282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GESTFF 9
           GEST+F
Sbjct: 843 GESTYF 848
>NP_055959
          Length = 949

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MSGESTF 8
           +SG+STF
Sbjct: 864 LSGQSTF 870
>NP_689450
          Length = 270

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MSGESTFF 9
          + G+STFF
Sbjct: 82 LPGKSTFF 89
>XP_006726562
          Length = 140

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_005276411
          Length = 150

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_005276410
          Length = 155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006726561
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_005276409
          Length = 172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_005276408
          Length = 178

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_005276407
          Length = 229

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006726559
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_005276406
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006726558
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006724974
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 76 GESTYF 81
>XP_005257319
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MSGESTFF 9
          + G+STFF
Sbjct: 74 LPGKSTFF 81
>XP_005257318
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MSGESTFF 9
          + G+STFF
Sbjct: 74 LPGKSTFF 81
>XP_005257317
          Length = 270

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MSGESTFF 9
          + G+STFF
Sbjct: 82 LPGKSTFF 89
>XP_005255915
          Length = 1099

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    MSGESTF 8
            +SG+STF
Sbjct: 1001 LSGQSTF 1007
>XP_005271906
          Length = 879

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GESTFF 9
           GEST+F
Sbjct: 843 GESTYF 848
>XP_006718144
          Length = 886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GESTFF 9
           GEST+F
Sbjct: 843 GESTYF 848
>XP_005271905
          Length = 1219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GESTFF 9
           GEST+F
Sbjct: 843 GESTYF 848
>XP_005271904
          Length = 1219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GESTFF 9
           GEST+F
Sbjct: 780 GESTYF 785
>XP_005271903
          Length = 1282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GESTFF 9
           GEST+F
Sbjct: 843 GESTYF 848
>XP_006716964
          Length = 155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006716963
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006716962
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>XP_006716961
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          GEST+F
Sbjct: 9  GESTYF 14
>NP_037455
          Length = 270

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MSGESTFF 9
          + G+STFF
Sbjct: 82 LPGKSTFF 89
>NP_037473
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GESTFF 9
          G+STFF
Sbjct: 34 GKSTFF 39
>NP_653081
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2 MSGESTFF 9
         M+GE  FF
Sbjct: 1 MNGEEEFF 8
>NP_055650
          Length = 468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2 MSGESTFF 9
         M+GE  FF
Sbjct: 1 MNGEEEFF 8
>NP_002431
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MSGESTF 8
           MSG+ST+
Sbjct: 683 MSGKSTY 689
>NP_001138590
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    TMSGESTF 8
            T+ G+STF
Sbjct: 1056 TVDGKSTF 1063
>XP_005257785
          Length = 1887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TMSGEST 7
           T+SG+ST
Sbjct: 258 TLSGDST 264
>XP_006718300
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   GESTFF 9
           GE TFF
Sbjct: 174 GEDTFF 179
>XP_006718299
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   GESTFF 9
           GE TFF
Sbjct: 214 GEDTFF 219
>XP_005252990
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   GESTFF 9
           GE TFF
Sbjct: 214 GEDTFF 219
>NP_004305
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   GESTFF 9
           GE TFF
Sbjct: 214 GEDTFF 219
>NP_006811
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SGESTFF 9
           SG+S+FF
Sbjct: 208 SGKSSFF 214
>NP_115510
          Length = 1663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  TMSGEST 7
          T+SG+ST
Sbjct: 92 TLSGDST 98
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.125    0.345 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 639,222
Number of extensions: 1041
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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