Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444284                                                              16    5631
XP_006718716                                                           16    5631
NP_060174                                                              16    7355
NP_000450                                                              15    9605
NP_001277006                                                           15    9605
NP_001269701                                                           15    9605
NP_061887                                                              15    9605
NP_001269703                                                           15    9605
NP_001269706                                                           15    9605
NP_001269708                                                           15    9605
NP_001269705                                                           15    9605
NP_001131076                                                           15    9605
XP_005268276                                                           15    9605
XP_005251620                                                           15    9605
XP_005251618                                                           15    9605
NP_004265                                                              15    9605
>NP_444284
          Length = 295

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MELILVN 8
           MEL+LVN
Sbjct: 140 MELLLVN 146
>XP_006718716
          Length = 303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MELILVN 8
           MEL+LVN
Sbjct: 148 MELLLVN 154
>NP_060174
          Length = 239

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TMELILVN 8
           T+EL+L+N
Sbjct: 190 TLELLLMN 197
>NP_000450
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MELILVN 8
          M+LIL+N
Sbjct: 24 MDLILIN 30
>NP_001277006
          Length = 1081

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MELILVN 8
          M+LIL+N
Sbjct: 24 MDLILIN 30
>NP_001269701
          Length = 1173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 674 TMEQIFMNV 682
>NP_061887
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 636 TMEQIFMNV 644
>NP_001269703
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 453 TMEQIFMNV 461
>NP_001269706
          Length = 996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 497 TMEQIFMNV 505
>NP_001269708
          Length = 996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 497 TMEQIFMNV 505
>NP_001269705
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 425 TMEQIFMNV 433
>NP_001131076
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMELILVNV 9
           TME I +NV
Sbjct: 580 TMEQIFMNV 588
>XP_005268276
          Length = 2320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    MELILVNV 9
            M+LI+VN+
Sbjct: 1164 MQLIIVNL 1171
>XP_005251620
          Length = 1080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MELILVN 8
          M+LIL+N
Sbjct: 24 MDLILIN 30
>XP_005251618
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MELILVN 8
          M+LIL+N
Sbjct: 24 MDLILIN 30
>NP_004265
          Length = 2319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    MELILVNV 9
            M+LI+VN+
Sbjct: 1163 MQLIIVNL 1170
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.142    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 361,650
Number of extensions: 380
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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