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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_919047                                                              19     665
XP_005250598                                                           19     665
NP_006100                                                              16    7355
NP_001034708                                                           16    7355
NP_001269885                                                           16    7355
XP_005255623                                                           15    9605
NP_061720                                                              15    9605
NP_076936                                                              15    9605

>NP_919047
          Length = 520

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TLVMGKVSQ 9
           TLVMGKVS+
Sbjct: 359 TLVMGKVSR 367



>XP_005250598
          Length = 457

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TLVMGKVSQ 9
           TLVMGKVS+
Sbjct: 296 TLVMGKVSR 304



>NP_006100
          Length = 637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TLVMGKVS 8
          TL++GK+S
Sbjct: 80 TLIVGKLS 87



>NP_001034708
          Length = 620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TLVMGKVS 8
          TL++GK+S
Sbjct: 63 TLIVGKLS 70



>NP_001269885
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TLVMGKVS 8
          TL++GK+S
Sbjct: 63 TLIVGKLS 70



>XP_005255623
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLVMGKVSQ 9
           T  MGKVS+
Sbjct: 437 TKTMGKVSR 445



>NP_061720
          Length = 4024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    TLVMGKVS 8
            TLV GK+S
Sbjct: 1222 TLVRGKLS 1229



>NP_076936
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLVMGKVSQ 9
           T  MGKVS+
Sbjct: 437 TKTMGKVSR 445



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.125    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 311,413
Number of extensions: 177
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)