Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_003632                                                              17    2528
XP_005258838                                                           17    2528
NP_775904                                                              17    2528
NP_061859                                                              16    5631
XP_006713954                                                           16    5631
XP_006713953                                                           16    5631
XP_006713952                                                           16    5631
XP_005248031                                                           16    5631
XP_006713951                                                           16    5631
NP_878908                                                              16    7355
NP_569733                                                              16    7355
NP_904324                                                              16    7355
NP_055501                                                              16    7355
NP_001275677                                                           16    7355
NP_061740                                                              16    7355
NP_114442                                                              16    7355
XP_006726924                                                           16    7355
XP_005259501                                                           16    7355
XP_006719745                                                           16    7355
XP_006719744                                                           16    7355
XP_005254044                                                           16    7355
NP_006454                                                              15    9605
NP_998787                                                              15    9605
NP_964010                                                              15    9605
NP_001182192                                                           15    9605
NP_079171                                                              15    9605
NP_001231                                                              15    9605
NP_490595                                                              15    9605
XP_006722961                                                           15    9605
XP_006718038                                                           15    9605
XP_005270222                                                           15    9605
XP_005263889                                                           15    9605
XP_006712890                                                           15    9605
XP_005263888                                                           15    9605
XP_005263887                                                           15    9605
XP_005264148                                                           15    9605
XP_005264145                                                           15    9605
XP_006711984                                                           15    9605
XP_006711983                                                           15    9605
NP_001232                                                              15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    LTHVMLQI 9
            LTHVMLQI
Sbjct: 2510 LTHVMLQI 2517
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    LTHVMLQI 9
            LTHVMLQI
Sbjct: 2305 LTHVMLQI 2312
>NP_003632
          Length = 125

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TLTHVMLQI 9
           T+ H+MLQI
Sbjct: 110 TVYHIMLQI 118
>XP_005258838
          Length = 563

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TLTHVMLQI 9
           TLTHV+L I
Sbjct: 302 TLTHVVLLI 310
>NP_775904
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TLTHVMLQI 9
           TLTHV+L I
Sbjct: 288 TLTHVVLLI 296
>NP_061859
          Length = 1336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   THVMLQI 9
           THV+LQ+
Sbjct: 946 THVLLQL 952
>XP_006713954
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   THVMLQI 9
           THV+LQ+
Sbjct: 653 THVLLQL 659
>XP_006713953
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   THVMLQI 9
           THV+LQ+
Sbjct: 653 THVLLQL 659
>XP_006713952
          Length = 1165

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   THVMLQI 9
           THV+LQ+
Sbjct: 775 THVLLQL 781
>XP_005248031
          Length = 1260

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   THVMLQI 9
           THV+LQ+
Sbjct: 870 THVLLQL 876
>XP_006713951
          Length = 1309

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   THVMLQI 9
           THV+LQ+
Sbjct: 919 THVLLQL 925
>NP_878908
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TLTHVML 7
           T THVML
Sbjct: 197 TKTHVML 203
>NP_569733
          Length = 1068

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           TLT + LQI
Sbjct: 473 TLTQIRLQI 481
>NP_904324
          Length = 1068

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           TLT + LQI
Sbjct: 473 TLTQIRLQI 481
>NP_055501
          Length = 1070

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   THVMLQI 9
           TH MLQ+
Sbjct: 368 THTMLQL 374
>NP_001275677
          Length = 604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           T+TH ML +
Sbjct: 503 TVTHFMLAV 511
>NP_061740
          Length = 962

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           T TH+ LQ+
Sbjct: 465 TETHISLQV 473
>NP_114442
          Length = 851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           T TH+ LQ+
Sbjct: 465 TETHISLQV 473
>XP_006726924
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TLTHVML 7
           T THVML
Sbjct: 183 TKTHVML 189
>XP_005259501
          Length = 1070

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   THVMLQI 9
           TH MLQ+
Sbjct: 368 THTMLQL 374
>XP_006719745
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           TLT + LQI
Sbjct: 473 TLTQIRLQI 481
>XP_006719744
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           TLT + LQI
Sbjct: 473 TLTQIRLQI 481
>XP_005254044
          Length = 1068

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLTHVMLQI 9
           TLT + LQI
Sbjct: 473 TLTQIRLQI 481
>NP_006454
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 295 TITHVLI 301
>NP_998787
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 295 TITHVLI 301
>NP_964010
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 295 TITHVLI 301
>NP_001182192
          Length = 1033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   THVMLQI 9
           TH+ML I
Sbjct: 282 THIMLTI 288
>NP_079171
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   THVMLQI 9
           TH+ML I
Sbjct: 282 THIMLTI 288
>NP_001231
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LTHVMLQI 9
           LTH  LQI
Sbjct: 237 LTHEFLQI 244
>NP_490595
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LTHVMLQI 9
           LTH  LQI
Sbjct: 236 LTHEFLQI 243
>XP_006722961
          Length = 214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TLTHVMLQ 8
          T+TH +LQ
Sbjct: 17 TMTHRLLQ 24
>XP_006718038
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   THVMLQI 9
           TH+ML I
Sbjct: 282 THIMLTI 288
>XP_005270222
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   THVMLQI 9
           TH+ML I
Sbjct: 282 THIMLTI 288
>XP_005263889
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  LTHVMLQI 9
          LTH  LQI
Sbjct: 61 LTHEFLQI 68
>XP_006712890
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  LTHVMLQI 9
          LTH  LQI
Sbjct: 92 LTHEFLQI 99
>XP_005263888
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  LTHVMLQI 9
          LTH  LQI
Sbjct: 92 LTHEFLQI 99
>XP_005263887
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LTHVMLQI 9
           LTH  LQI
Sbjct: 215 LTHEFLQI 222
>XP_005264148
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 160 TITHVLI 166
>XP_005264145
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 295 TITHVLI 301
>XP_006711984
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 342 TITHVLI 348
>XP_006711983
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TLTHVML 7
           T+THV++
Sbjct: 342 TITHVLI 348
>NP_001232
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LTHVMLQI 9
           LTH  LQI
Sbjct: 236 LTHEFLQI 243
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.138    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 528,807
Number of extensions: 814
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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