Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           19     665
NP_060785                                                              19     665
NP_361014                                                              19     665
NP_000578                                                              17    2528
NP_001185739                                                           17    4312
NP_003618                                                              17    4312
NP_001361                                                              17    4312
XP_005274415                                                           17    4312
XP_005274414                                                           17    4312
XP_006712019                                                           17    4312
NP_001080                                                              16    5631
NP_848599                                                              16    5631
NP_001157164                                                           16    5631
XP_005254427                                                           16    5631
XP_006713081                                                           16    5631
NP_085116                                                              16    7355
NP_000005                                                              16    7355
XP_006719119                                                           16    7355
NP_001108451                                                           15    9605
NP_001108454                                                           15    9605
NP_003713                                                              15    9605
NP_001108450                                                           15    9605
NP_001108452                                                           15    9605
NP_001108453                                                           15    9605
NP_060379                                                              15    9605
NP_060232                                                              15    9605
XP_006725610                                                           15    9605
XP_006725609                                                           15    9605
XP_006725608                                                           15    9605
XP_005254565                                                           15    9605
XP_005254564                                                           15    9605
XP_006720651                                                           15    9605
XP_005247903                                                           15    9605
XP_005247901                                                           15    9605
XP_005247900                                                           15    9605
>NP_001013433
          Length = 589

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TLTGRQSLT 9
           TLTG QSLT
Sbjct: 458 TLTGHQSLT 466
>NP_060785
          Length = 627

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TLTGRQSLT 9
           TLTG QSLT
Sbjct: 496 TLTGHQSLT 504
>NP_361014
          Length = 707

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TLTGRQSLT 9
           TLTG QSLT
Sbjct: 576 TLTGHQSLT 584
>NP_000578
          Length = 843

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TLTGRQSLT 9
           TLTGR S T
Sbjct: 756 TLTGRDSCT 764
>NP_001185739
          Length = 559

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           TLTG QSL
Sbjct: 478 TLTGLQSL 485
>NP_003618
          Length = 559

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           TLTG QSL
Sbjct: 478 TLTGLQSL 485
>NP_001361
          Length = 4158

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TGRQSLT 9
            TG+QSLT
Sbjct: 2451 TGKQSLT 2457
>XP_005274415
          Length = 575

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           TLTG QSL
Sbjct: 494 TLTGLQSL 501
>XP_005274414
          Length = 585

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           TLTG QSL
Sbjct: 504 TLTGLQSL 511
>XP_006712019
          Length = 4158

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TGRQSLT 9
            TG+QSLT
Sbjct: 2451 TGKQSLT 2457
>NP_001080
          Length = 1704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LTGRQSLT 9
            LTG +SLT
Sbjct: 1429 LTGEESLT 1436
>NP_848599
          Length = 3092

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TGRQSLT 9
            +GRQSLT
Sbjct: 2261 SGRQSLT 2267
>NP_001157164
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TGRQSLT 9
           TGRQSL+
Sbjct: 296 TGRQSLS 302
>XP_005254427
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TGRQSLT 9
           TGRQSL+
Sbjct: 296 TGRQSLS 302
>XP_006713081
          Length = 3964

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TGRQSLT 9
            +GRQSLT
Sbjct: 2284 SGRQSLT 2290
>NP_085116
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TGRQSLT 9
           TGR SLT
Sbjct: 364 TGRSSLT 370
>NP_000005
          Length = 1474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LTGRQS 7
           LTGRQS
Sbjct: 335 LTGRQS 340
>XP_006719119
          Length = 1512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LTGRQS 7
           LTGRQS
Sbjct: 335 LTGRQS 340
>NP_001108451
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 280 ITGRQSV 286
>NP_001108454
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 186 ITGRQSV 192
>NP_003713
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 280 ITGRQSV 286
>NP_001108450
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 280 ITGRQSV 286
>NP_001108452
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 186 ITGRQSV 192
>NP_001108453
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 186 ITGRQSV 192
>NP_060379
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TLTGRQSL 8
          TLT RQSL
Sbjct: 43 TLTIRQSL 50
>NP_060232
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 427 TITGFQSL 434
>XP_006725610
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 179 TITGFQSL 186
>XP_006725609
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 427 TITGFQSL 434
>XP_006725608
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 427 TITGFQSL 434
>XP_005254565
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 179 TITGFQSL 186
>XP_005254564
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 427 TITGFQSL 434
>XP_006720651
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TLTGRQSL 8
           T+TG QSL
Sbjct: 427 TITGFQSL 434
>XP_005247903
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 280 ITGRQSV 286
>XP_005247901
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 263 ITGRQSV 269
>XP_005247900
          Length = 676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTGRQSL 8
           +TGRQS+
Sbjct: 280 ITGRQSV 286
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.121    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 412,553
Number of extensions: 426
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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