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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              18    1482
NP_005432                                                              16    5631
NP_004851                                                              16    5631
NP_008958                                                              16    7355
NP_006763                                                              15    9605

>NP_005179
          Length = 906

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TLSDISTAS 9
           TLSDIS AS
Sbjct: 789 TLSDISNAS 797



>NP_005432
          Length = 559

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TLSDISTAS 9
           TLSDIS +S
Sbjct: 348 TLSDISWSS 356



>NP_004851
          Length = 673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SDISTAS 9
           SD+STAS
Sbjct: 447 SDVSTAS 453



>NP_008958
          Length = 1522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TLSDISTAS 9
           TLSD+S  S
Sbjct: 310 TLSDVSNIS 318



>NP_006763
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSDISTA 8
           L+DISTA
Sbjct: 718 LNDISTA 724



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.103    0.226 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 305,401
Number of extensions: 277
Number of successful extensions: 5
Number of sequences better than 10000.0: 5
Number of HSP's gapped: 5
Number of HSP's successfully gapped: 5
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)