Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443200                                                              17    2528
NP_001160070                                                           17    2528
XP_006718824                                                           17    2528
NP_667338                                                              16    7355
NP_001159608                                                           16    7355
NP_001268885                                                           16    7355
NP_443128                                                              16    7355
XP_006713923                                                           16    7355
XP_006713922                                                           16    7355
NP_002560                                                              15    9605
NP_001276753                                                           15    9605
NP_001276752                                                           15    9605
NP_055698                                                              15    9605
NP_001015045                                                           15    9605
NP_001252509                                                           15    9605
NP_001252508                                                           15    9605
NP_001252507                                                           15    9605
XP_005262351                                                           15    9605
XP_005262742                                                           15    9605
XP_005262741                                                           15    9605
XP_006714120                                                           15    9605
XP_005262740                                                           15    9605
XP_005262739                                                           15    9605
XP_005262738                                                           15    9605
>NP_443200
          Length = 366

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  LSAYSATQ 9
          LSA+SATQ
Sbjct: 15 LSAFSATQ 22
>NP_001160070
          Length = 366

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  LSAYSATQ 9
          LSA+SATQ
Sbjct: 15 LSAFSATQ 22
>XP_006718824
          Length = 366

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  LSAYSATQ 9
          LSA+SATQ
Sbjct: 15 LSAFSATQ 22
>NP_667338
          Length = 1308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TLSAYSA 7
           TLSAY+A
Sbjct: 981 TLSAYTA 987
>NP_001159608
          Length = 1228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TLSAYSA 7
           TLSAY+A
Sbjct: 901 TLSAYTA 907
>NP_001268885
          Length = 3631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TLSAYSAT 8
            TLS Y AT
Sbjct: 2670 TLSVYGAT 2677
>NP_443128
          Length = 3487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    TLSAYSAT 8
            TLS Y AT
Sbjct: 2672 TLSVYGAT 2679
>XP_006713923
          Length = 1228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TLSAYSA 7
           TLSAY+A
Sbjct: 901 TLSAYTA 907
>XP_006713922
          Length = 1262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TLSAYSA 7
           TLSAY+A
Sbjct: 981 TLSAYTA 987
>NP_002560
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TLSAYSATQ 9
           TLS  SATQ
Sbjct: 314 TLSISSATQ 322
>NP_001276753
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TLSAYSATQ 9
           TLS  SATQ
Sbjct: 314 TLSISSATQ 322
>NP_001276752
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TLSAYSATQ 9
           TLS  SATQ
Sbjct: 314 TLSISSATQ 322
>NP_055698
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 396 TLSASSAT 403
>NP_001015045
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TLSAYSAT 8
          TLSA SAT
Sbjct: 70 TLSASSAT 77
>NP_001252509
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TLSAYSAT 8
          TLSA SAT
Sbjct: 42 TLSASSAT 49
>NP_001252508
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TLSAYSAT 8
          TLSA SAT
Sbjct: 70 TLSASSAT 77
>NP_001252507
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TLSAYSAT 8
          TLSA SAT
Sbjct: 56 TLSASSAT 63
>XP_005262351
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LSAYSAT 8
           L AYSAT
Sbjct: 389 LQAYSAT 395
>XP_005262742
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 396 TLSASSAT 403
>XP_005262741
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 187 TLSASSAT 194
>XP_006714120
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 207 TLSASSAT 214
>XP_005262740
          Length = 995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 396 TLSASSAT 403
>XP_005262739
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 376 TLSASSAT 383
>XP_005262738
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLSAYSAT 8
           TLSA SAT
Sbjct: 382 TLSASSAT 389
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296   0.0986    0.231 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 337,251
Number of extensions: 489
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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