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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              18    1482
NP_004163                                                              16    5631
NP_001160167                                                           16    5631
NP_001276868                                                           16    5631
NP_001276869                                                           16    5631
XP_006714551                                                           16    5631
XP_005248399                                                           16    5631
NP_001136024                                                           16    7355
NP_001438                                                              16    7355
XP_006714824                                                           16    7355
NP_001239268                                                           15    9605
NP_001239267                                                           15    9605
NP_001785                                                              15    9605

>NP_004351
          Length = 882

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TLKVTAADA 9
           TLKVT ADA
Sbjct: 395 TLKVTDADA 403



>NP_004163
          Length = 542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           ++VTAADA
Sbjct: 162 VRVTAADA 169



>NP_001160167
          Length = 497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           ++VTAADA
Sbjct: 162 VRVTAADA 169



>NP_001276868
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           ++VTAADA
Sbjct: 116 VRVTAADA 123



>NP_001276869
          Length = 430

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           ++VTAADA
Sbjct: 162 VRVTAADA 169



>XP_006714551
          Length = 542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           ++VTAADA
Sbjct: 162 VRVTAADA 169



>XP_005248399
          Length = 542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           ++VTAADA
Sbjct: 162 VRVTAADA 169



>NP_001136024
          Length = 1369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKVTAADA 9
           L+VTA+DA
Sbjct: 683 LQVTASDA 690



>NP_001438
          Length = 4349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TLKVTAAD 8
           +LK+TA+D
Sbjct: 637 SLKITASD 644



>XP_006714824
          Length = 4349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TLKVTAAD 8
           +LK+TA+D
Sbjct: 637 SLKITASD 644



>NP_001239268
          Length = 842

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LKVTAADA 9
           + +TAADA
Sbjct: 557 INITAADA 564



>NP_001239267
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LKVTAADA 9
           + +TAADA
Sbjct: 594 INITAADA 601



>NP_001785
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LKVTAADA 9
           + +TAADA
Sbjct: 631 INITAADA 638



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.112    0.266 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 226,076
Number of extensions: 240
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)