Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              17    2528
NP_671714                                                              17    2528
XP_006715890                                                           17    2528
XP_005250160                                                           17    2528
XP_005250159                                                           17    2528
NP_001106677                                                           17    4312
NP_055668                                                              16    5631
XP_006721677                                                           16    5631
XP_006721676                                                           16    5631
NP_001091979                                                           16    5631
NP_055495                                                              16    7355
NP_065987                                                              16    7355
NP_005039                                                              16    7355
XP_006722261                                                           16    7355
XP_005258130                                                           16    7355
NP_001275635                                                           15    9605
NP_001264156                                                           15    9605
NP_003658                                                              15    9605
NP_001264155                                                           15    9605
NP_000868                                                              15    9605
NP_055537                                                              15    9605
NP_001273374                                                           15    9605
XP_006715343                                                           15    9605
XP_006715342                                                           15    9605
XP_006715341                                                           15    9605
XP_006715340                                                           15    9605
XP_006715339                                                           15    9605
XP_006715338                                                           15    9605
XP_005263991                                                           15    9605
XP_005263989                                                           15    9605
XP_005263990                                                           15    9605
XP_005263988                                                           15    9605
XP_005263987                                                           15    9605
XP_005263986                                                           15    9605

>NP_005742
          Length = 3907

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TLGISELES 9
            TL ISELES
Sbjct: 2605 TLRISELES 2613



>NP_671714
          Length = 3899

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TLGISELES 9
            TL ISELES
Sbjct: 2597 TLRISELES 2605



>XP_006715890
          Length = 3860

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TLGISELES 9
            TL ISELES
Sbjct: 2558 TLRISELES 2566



>XP_005250160
          Length = 3939

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TLGISELES 9
            TL ISELES
Sbjct: 2637 TLRISELES 2645



>XP_005250159
          Length = 3952

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TLGISELES 9
            TL ISELES
Sbjct: 2650 TLRISELES 2658



>NP_001106677
          Length = 74

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 GISELES 9
         G+SELES
Sbjct: 3 GVSELES 9



>NP_055668
          Length = 1051

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLGISELE 8
           TLG SELE
Sbjct: 302 TLGDSELE 309



>XP_006721677
          Length = 1028

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLGISELE 8
           TLG SELE
Sbjct: 302 TLGDSELE 309



>XP_006721676
          Length = 1073

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLGISELE 8
           TLG SELE
Sbjct: 302 TLGDSELE 309



>NP_001091979
          Length = 1006

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TLGISELE 8
           TLG SELE
Sbjct: 302 TLGDSELE 309



>NP_055495
          Length = 2235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    LGISELE 8
            LGI+ELE
Sbjct: 1927 LGIAELE 1933



>NP_065987
          Length = 600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LGISELE 8
           LGIS+LE
Sbjct: 591 LGISDLE 597



>NP_005039
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           +G+ ELES
Sbjct: 205 IGVKELES 212



>XP_006722261
          Length = 2092

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    LGISELE 8
            LGI+ELE
Sbjct: 1784 LGIAELE 1790



>XP_005258130
          Length = 2234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    LGISELE 8
            LGI+ELE
Sbjct: 1926 LGIAELE 1932



>NP_001275635
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>NP_001264156
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LGISELES 9
          LG+SEL S
Sbjct: 55 LGLSELPS 62



>NP_003658
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LGISELES 9
          LG+SEL S
Sbjct: 55 LGLSELPS 62



>NP_001264155
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LGISELES 9
          LG+SEL S
Sbjct: 55 LGLSELPS 62



>NP_000868
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>NP_055537
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 895 GVSDLES 901



>NP_001273374
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 874 GVSDLES 880



>XP_006715343
          Length = 1018

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 845 GVSDLES 851



>XP_006715342
          Length = 1018

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 845 GVSDLES 851



>XP_006715341
          Length = 1018

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 845 GVSDLES 851



>XP_006715340
          Length = 1018

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 845 GVSDLES 851



>XP_006715339
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 857 GVSDLES 863



>XP_006715338
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GISELES 9
           G+S+LES
Sbjct: 879 GVSDLES 885



>XP_005263991
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>XP_005263989
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>XP_005263990
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>XP_005263988
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>XP_005263987
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



>XP_005263986
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGISELES 9
           L ISE+ES
Sbjct: 296 LNISEIES 303



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.123    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 480,219
Number of extensions: 740
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)