Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001166028                                                           17    4312
NP_005886                                                              17    4312
XP_006719803                                                           17    4312
XP_005266222                                                           17    4312
XP_005266221                                                           17    4312
XP_006719802                                                           17    4312
XP_006719801                                                           17    4312
XP_006719800                                                           17    4312
XP_006719799                                                           17    4312
XP_005266219                                                           17    4312
NP_057652                                                              16    7355
NP_064714                                                              16    7355
XP_005245479                                                           16    7355
NP_068755                                                              16    7355
NP_003283                                                              15    9605
NP_001028732                                                           15    9605
NP_036292                                                              15    9605
NP_001265645                                                           15    9605
NP_001243467                                                           15    9605
NP_570722                                                              15    9605
NP_001028731                                                           15    9605
XP_005266987                                                           15    9605
XP_006715521                                                           15    9605
XP_005245528                                                           15    9605
NP_542777                                                              15    9605
>NP_001166028
          Length = 1134

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 179 TKTRLFST 186
>NP_005886
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 179 TKTRLFST 186
>XP_006719803
          Length = 1407

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  TKTAIFST 8
          TKT +FST
Sbjct: 88 TKTRLFST 95
>XP_005266222
          Length = 1458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 139 TKTRLFST 146
>XP_005266221
          Length = 1458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 139 TKTRLFST 146
>XP_006719802
          Length = 1458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 139 TKTRLFST 146
>XP_006719801
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 179 TKTRLFST 186
>XP_006719800
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 179 TKTRLFST 186
>XP_006719799
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 179 TKTRLFST 186
>XP_005266219
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           TKT +FST
Sbjct: 179 TKTRLFST 186
>NP_057652
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TAIFSTT 9
           +AIFSTT
Sbjct: 232 SAIFSTT 238
>NP_064714
          Length = 1791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           T TAIF+T
Sbjct: 172 TTTAIFTT 179
>XP_005245479
          Length = 1791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           T TAIF+T
Sbjct: 172 TTTAIFTT 179
>NP_068755
          Length = 827

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKTAIFST 8
           T TAIF+T
Sbjct: 172 TTTAIFTT 179
>NP_003283
          Length = 2363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    TKTAIFST 8
            T TA+F T
Sbjct: 1811 TSTAVFGT 1818
>NP_001028732
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TKTAIF 6
          TKTA+F
Sbjct: 13 TKTAVF 18
>NP_036292
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKTAIFST 8
          T+TA+F T
Sbjct: 92 TQTAVFRT 99
>NP_001265645
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKTAIFST 8
          T+TA+F T
Sbjct: 92 TQTAVFRT 99
>NP_001243467
          Length = 165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TKTAIFSTT 9
          T  A+FSTT
Sbjct: 60 TIQAVFSTT 68
>NP_570722
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TKTAIF 6
          TKTA+F
Sbjct: 13 TKTAVF 18
>NP_001028731
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TKTAIF 6
          TKTA+F
Sbjct: 13 TKTAVF 18
>XP_005266987
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKTAIFST 8
          T+TA+F T
Sbjct: 92 TQTAVFRT 99
>XP_006715521
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKTAIFST 8
          T+TA+F T
Sbjct: 92 TQTAVFRT 99
>XP_005245528
          Length = 2439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    TKTAIFST 8
            T TA+F T
Sbjct: 1887 TSTAVFGT 1894
>NP_542777
          Length = 165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TKTAIFSTT 9
          T  A+FSTT
Sbjct: 60 TIQAVFSTT 68
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.111    0.274 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 309,812
Number of extensions: 550
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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