Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     390
NP_858045                                                              20     390
NP_006525                                                              20     390
NP_001167559                                                           20     390
NP_078966                                                              17    3301
NP_919263                                                              17    3301
XP_006723004                                                           17    3301
XP_006720118                                                           17    3301
XP_006720117                                                           17    3301
XP_006720116                                                           17    3301
XP_005267417                                                           17    3301
NP_000954                                                              17    4312
NP_057396                                                              17    4312
NP_001265506                                                           16    5631
NP_001265507                                                           16    5631
NP_001265505                                                           16    5631
NP_001265504                                                           16    5631
NP_001192202                                                           16    5631
NP_001003801                                                           16    7355
NP_112174                                                              16    7355
NP_001018098                                                           15    9605
NP_060154                                                              15    9605
NP_060550                                                              15    9605
NP_065872                                                              15    9605
>NP_001167558
          Length = 1423

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TKSKLFLNP 9
           TKSKLF NP
Sbjct: 347 TKSKLFRNP 355
>NP_858045
          Length = 1424

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TKSKLFLNP 9
           TKSKLF NP
Sbjct: 347 TKSKLFRNP 355
>NP_006525
          Length = 1420

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TKSKLFLNP 9
           TKSKLF NP
Sbjct: 347 TKSKLFRNP 355
>NP_001167559
          Length = 1415

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TKSKLFLNP 9
           TKSKLF NP
Sbjct: 357 TKSKLFRNP 365
>NP_078966
          Length = 14507

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    TKSKLFLNP 9
            TKSKL L P
Sbjct: 9280 TKSKLSLTP 9288
>NP_919263
          Length = 488

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KLFLNP 9
           KLFLNP
Sbjct: 336 KLFLNP 341
>XP_006723004
          Length = 6443

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    TKSKLFLNP 9
            TKSKL L P
Sbjct: 1194 TKSKLSLTP 1202
>XP_006720118
          Length = 248

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KLFLNP 9
           KLFLNP
Sbjct: 96  KLFLNP 101
>XP_006720117
          Length = 396

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KLFLNP 9
           KLFLNP
Sbjct: 244 KLFLNP 249
>XP_006720116
          Length = 396

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KLFLNP 9
           KLFLNP
Sbjct: 244 KLFLNP 249
>XP_005267417
          Length = 471

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KLFLNP 9
           KLFLNP
Sbjct: 319 KLFLNP 324
>NP_000954
          Length = 604

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TKSKLFLNP 9
          T+ KLFL P
Sbjct: 61 TRIKLFLKP 69
>NP_057396
          Length = 641

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TKSKLFLNP 9
          TKS  F+NP
Sbjct: 9  TKSGKFMNP 17
>NP_001265506
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKSKLFL 7
           TK+KLFL
Sbjct: 105 TKAKLFL 111
>NP_001265507
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKSKLFL 7
           TK+KLFL
Sbjct: 105 TKAKLFL 111
>NP_001265505
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKSKLFL 7
           TK+KLFL
Sbjct: 105 TKAKLFL 111
>NP_001265504
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKSKLFL 7
           TK+KLFL
Sbjct: 105 TKAKLFL 111
>NP_001192202
          Length = 148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKSKLFL 7
           TK+KLFL
Sbjct: 113 TKAKLFL 119
>NP_001003801
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  TKSKLF 6
          TKSKLF
Sbjct: 14 TKSKLF 19
>NP_112174
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKLFLN 8
           SKLFLN
Sbjct: 330 SKLFLN 335
>NP_001018098
          Length = 3628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SKLFLNP 9
           +KL++NP
Sbjct: 303 AKLYMNP 309
>NP_060154
          Length = 3710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SKLFLNP 9
           +KL++NP
Sbjct: 260 AKLYMNP 266
>NP_060550
          Length = 3585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SKLFLNP 9
           +KL++NP
Sbjct: 260 AKLYMNP 266
>NP_065872
          Length = 3753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SKLFLNP 9
           +KL++NP
Sbjct: 303 AKLYMNP 309
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.132    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 532,672
Number of extensions: 1070
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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