Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002515                                                              19     869
NP_001107228                                                           17    2528
NP_001244918                                                           17    2528
NP_001107227                                                           17    2528
NP_001944                                                              17    2528
NP_001244917                                                           17    2528
NP_006377                                                              17    3301
NP_001091974                                                           17    3301
NP_003144                                                              16    7355
NP_001171549                                                           16    7355
NP_001171550                                                           16    7355
NP_004154                                                              16    7355
NP_001171551                                                           16    7355
NP_001171552                                                           16    7355
XP_006722581                                                           16    7355
XP_005266791                                                           16    7355
XP_005266790                                                           16    7355
XP_006719638                                                           16    7355
XP_006719637                                                           16    7355
XP_006719636                                                           16    7355
NP_699207                                                              16    7355
NP_006378                                                              15    9605
>NP_002515
          Length = 189

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TKQARELAK 9
           TKQA ELAK
Sbjct: 127 TKQAHELAK 135
>NP_001107228
          Length = 482

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KQARELAK 9
           +QARELAK
Sbjct: 246 EQARELAK 253
>NP_001244918
          Length = 487

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KQARELAK 9
           +QARELAK
Sbjct: 246 EQARELAK 253
>NP_001107227
          Length = 482

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KQARELAK 9
           +QARELAK
Sbjct: 246 EQARELAK 253
>NP_001944
          Length = 482

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KQARELAK 9
           +QARELAK
Sbjct: 246 EQARELAK 253
>NP_001244917
          Length = 482

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KQARELAK 9
           +QARELAK
Sbjct: 246 EQARELAK 253
>NP_006377
          Length = 729

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KQARELAK 9
           KQAREL K
Sbjct: 528 KQARELIK 535
>NP_001091974
          Length = 731

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KQARELAK 9
           KQAREL K
Sbjct: 528 KQARELIK 535
>NP_003144
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 319 KQARELS 325
>NP_001171549
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 319 KQARELS 325
>NP_001171550
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 319 KQARELS 325
>NP_004154
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           +QARELA
Sbjct: 146 RQARELA 152
>NP_001171551
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 209 KQARELS 215
>NP_001171552
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 209 KQARELS 215
>XP_006722581
          Length = 182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           +QARELA
Sbjct: 146 RQARELA 152
>XP_005266791
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           +QARELA
Sbjct: 146 RQARELA 152
>XP_005266790
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           +QARELA
Sbjct: 146 RQARELA 152
>XP_006719638
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 319 KQARELS 325
>XP_006719637
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 319 KQARELS 325
>XP_006719636
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KQARELA 8
           KQAREL+
Sbjct: 319 KQARELS 325
>NP_699207
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKQARE 6
           TKQARE
Sbjct: 481 TKQARE 486
>NP_006378
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KQAREL 7
           KQAREL
Sbjct: 228 KQAREL 233
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.111    0.254 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 360,177
Number of extensions: 598
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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