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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004324                                                              19     665
XP_005250103                                                           19     665
XP_005250102                                                           19     665
NP_001268231                                                           16    7355
NP_115756                                                              16    7355
NP_733781                                                              16    7355
NP_849144                                                              16    7355
XP_006714784                                                           16    7355
XP_006713662                                                           16    7355
XP_006713661                                                           16    7355
XP_006713660                                                           16    7355
XP_005247434                                                           16    7355
XP_006713659                                                           16    7355
XP_006711559                                                           16    7355
XP_006711558                                                           16    7355
NP_001177255                                                           15    9605
NP_073557                                                              15    9605
NP_115901                                                              15    9605
NP_001275631                                                           15    9605
NP_699169                                                              15    9605
NP_001116362                                                           15    9605
XP_006724331                                                           15    9605
XP_005254804                                                           15    9605
XP_006720791                                                           15    9605
XP_005254803                                                           15    9605
XP_005254800                                                           15    9605
XP_005254799                                                           15    9605
XP_005274116                                                           15    9605
XP_006718652                                                           15    9605
XP_005274115                                                           15    9605
XP_005274114                                                           15    9605
NP_003913                                                              15    9605

>NP_004324
          Length = 766

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKPQLAIV 8
           TKPQLAIV
Sbjct: 521 TKPQLAIV 528



>XP_005250103
          Length = 758

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKPQLAIV 8
           TKPQLAIV
Sbjct: 521 TKPQLAIV 528



>XP_005250102
          Length = 767

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKPQLAIV 8
           TKPQLAIV
Sbjct: 521 TKPQLAIV 528



>NP_001268231
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KPQLAI 7
           KPQLAI
Sbjct: 426 KPQLAI 431



>NP_115756
          Length = 779

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KPQLAI 7
           KPQLAI
Sbjct: 394 KPQLAI 399



>NP_733781
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KPQLAI 7
           KPQLAI
Sbjct: 394 KPQLAI 399



>NP_849144
          Length = 2623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            TKP + I+A
Sbjct: 1186 TKPPMTIIA 1194



>XP_006714784
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KPQLAI 7
           KPQLAI
Sbjct: 394 KPQLAI 399



>XP_006713662
          Length = 1846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            TKP + I+A
Sbjct: 1258 TKPPMTIIA 1266



>XP_006713661
          Length = 1860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            TKP + I+A
Sbjct: 1258 TKPPMTIIA 1266



>XP_006713660
          Length = 2623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            TKP + I+A
Sbjct: 1186 TKPPMTIIA 1194



>XP_005247434
          Length = 2623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            TKP + I+A
Sbjct: 1186 TKPPMTIIA 1194



>XP_006713659
          Length = 2695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            TKP + I+A
Sbjct: 1258 TKPPMTIIA 1266



>XP_006711559
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KPQLAIV 8
           KP+LAIV
Sbjct: 214 KPRLAIV 220



>XP_006711558
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KPQLAIV 8
           KP+LAIV
Sbjct: 214 KPRLAIV 220



>NP_001177255
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KPQLAIVA 9
           KP+++IVA
Sbjct: 719 KPKMSIVA 726



>NP_073557
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KPQLAIVA 9
           KP+++IVA
Sbjct: 752 KPKMSIVA 759



>NP_115901
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TKPQLAIVA 9
           +KP+L I+A
Sbjct: 714 SKPELNIIA 722



>NP_001275631
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TKPQLAIV 8
           T PQLA V
Sbjct: 256 TNPQLAAV 263



>NP_699169
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TKPQLAIV 8
           T PQLA V
Sbjct: 218 TNPQLAAV 225



>NP_001116362
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TKPQLAIV 8
           T PQLA V
Sbjct: 250 TNPQLAAV 257



>XP_006724331
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KPQLAIVA 9
           KP+++IVA
Sbjct: 395 KPKMSIVA 402



>XP_005254804
          Length = 4796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            T PQ +I+A
Sbjct: 1553 TSPQASIIA 1561



>XP_006720791
          Length = 4853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            T PQ +I+A
Sbjct: 1618 TSPQASIIA 1626



>XP_005254803
          Length = 4854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            T PQ +I+A
Sbjct: 1618 TSPQASIIA 1626



>XP_005254800
          Length = 4861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            T PQ +I+A
Sbjct: 1618 TSPQASIIA 1626



>XP_005254799
          Length = 4861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            T PQ +I+A
Sbjct: 1618 TSPQASIIA 1626



>XP_005274116
          Length = 1602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKPQLA 6
           TKPQ+A
Sbjct: 283 TKPQIA 288



>XP_006718652
          Length = 2196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKPQLA 6
           TKPQ+A
Sbjct: 853 TKPQIA 858



>XP_005274115
          Length = 2222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKPQLA 6
           TKPQ+A
Sbjct: 879 TKPQIA 884



>XP_005274114
          Length = 2223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKPQLA 6
           TKPQ+A
Sbjct: 880 TKPQIA 885



>NP_003913
          Length = 4861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TKPQLAIVA 9
            T PQ +I+A
Sbjct: 1618 TSPQASIIA 1626



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.127    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 703,759
Number of extensions: 577
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)