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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     509
NP_112477                                                              16    7355
NP_001243298                                                           16    7355
NP_071429                                                              16    7355
NP_006632                                                              16    7355
NP_078932                                                              15    9605
XP_005260137                                                           15    9605

>NP_004110
          Length = 993

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TKMFMLNIR 9
           TKMF LNIR
Sbjct: 428 TKMFTLNIR 436



>NP_112477
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKMFMLNI 8
          T MF+LN+
Sbjct: 83 TDMFLLNL 90



>NP_001243298
          Length = 357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKMFMLNI 8
          T MF+LN+
Sbjct: 71 TDMFLLNL 78



>NP_071429
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKMFMLNI 8
          T MF+LN+
Sbjct: 78 TNMFILNL 85



>NP_006632
          Length = 357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TKMFMLNI 8
          T MF+LN+
Sbjct: 71 TDMFLLNL 78



>NP_078932
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KMFMLNIR 9
           ++FM+N+R
Sbjct: 342 EVFMINLR 349



>XP_005260137
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KMFMLNIR 9
           ++FM+N+R
Sbjct: 259 EVFMINLR 266



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.146    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 277,896
Number of extensions: 403
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)