Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     390
NP_001035957                                                           20     390
XP_006721991                                                           20     390
XP_006721990                                                           20     390
XP_006721989                                                           20     390
XP_005258041                                                           20     390
XP_006721988                                                           20     390
XP_006721987                                                           20     390
XP_006721986                                                           20     390
XP_005258040                                                           20     390
XP_006721985                                                           20     390
NP_612350                                                              18    1482
NP_001180382                                                           18    1482
NP_001180460                                                           18    1482
XP_006719743                                                           18    1482
XP_006719742                                                           18    1482
XP_006719741                                                           18    1482
XP_005254043                                                           18    1482
XP_005254042                                                           18    1482
NP_001258593                                                           17    3301
NP_001138476                                                           17    3301
NP_001001413                                                           17    3301
XP_006726626                                                           17    3301
XP_006726624                                                           17    3301
XP_006726623                                                           17    3301
XP_006725630                                                           17    3301
XP_006725629                                                           17    3301
XP_006725599                                                           17    3301
XP_006726291                                                           17    3301
XP_006726290                                                           17    3301
XP_006725238                                                           17    3301
XP_006720863                                                           17    3301
XP_006720862                                                           17    3301
XP_006720860                                                           17    3301
XP_006720859                                                           17    3301
XP_006720858                                                           17    3301
XP_006720426                                                           17    3301
NP_005496                                                              16    5631
NP_001076428                                                           16    5631
NP_689590                                                              16    7355
NP_001229831                                                           16    7355
NP_001229832                                                           16    7355
NP_003479                                                              16    7355
XP_006722190                                                           16    7355
XP_005257766                                                           16    7355
XP_005257764                                                           16    7355
XP_006722189                                                           16    7355
NP_005992                                                              15    9605
NP_525125                                                              15    9605
NP_001180343                                                           15    9605
NP_112492                                                              15    9605
NP_997195                                                              15    9605
NP_061816                                                              15    9605
>NP_000258
          Length = 2818

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 685 TKLEVALYM 693
>NP_001035957
          Length = 2839

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 685 TKLEVALYM 693
>XP_006721991
          Length = 1632

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 695 TKLEVALYM 703
>XP_006721990
          Length = 2823

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 695 TKLEVALYM 703
>XP_006721989
          Length = 2823

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 695 TKLEVALYM 703
>XP_005258041
          Length = 2836

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 685 TKLEVALYM 693
>XP_006721988
          Length = 2846

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 695 TKLEVALYM 703
>XP_006721987
          Length = 2849

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 695 TKLEVALYM 703
>XP_006721986
          Length = 2854

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 682 TKLEVALYM 690
>XP_005258040
          Length = 2857

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 685 TKLEVALYM 693
>XP_006721985
          Length = 2867

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKLEMALYM 9
           TKLE+ALYM
Sbjct: 695 TKLEVALYM 703
>NP_612350
          Length = 245

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 167 TKLHMALYV 175
>NP_001180382
          Length = 302

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 224 TKLHMALYV 232
>NP_001180460
          Length = 337

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 259 TKLHMALYV 267
>XP_006719743
          Length = 245

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 167 TKLHMALYV 175
>XP_006719742
          Length = 336

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 258 TKLHMALYV 266
>XP_006719741
          Length = 364

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 286 TKLHMALYV 294
>XP_005254043
          Length = 371

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 293 TKLHMALYV 301
>XP_005254042
          Length = 394

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TKLEMALYM 9
           TKL MALY+
Sbjct: 316 TKLHMALYV 324
>NP_001258593
          Length = 670

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 128 TELQMALY 135
>NP_001138476
          Length = 724

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 128 TELQMALY 135
>NP_001001413
          Length = 668

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 140 TELQMALY 147
>XP_006726626
          Length = 678

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 116 TELQMALY 123
>XP_006726624
          Length = 564

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006726623
          Length = 564

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006725630
          Length = 564

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006725629
          Length = 564

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006725599
          Length = 466

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 140 TELQMALY 147
>XP_006726291
          Length = 624

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006726290
          Length = 564

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006725238
          Length = 466

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 140 TELQMALY 147
>XP_006720863
          Length = 564

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 122 TELQMALY 129
>XP_006720862
          Length = 570

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 128 TELQMALY 135
>XP_006720860
          Length = 434

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 116 TELQMALY 123
>XP_006720859
          Length = 603

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 116 TELQMALY 123
>XP_006720858
          Length = 651

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 116 TELQMALY 123
>XP_006720426
          Length = 466

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TKLEMALY 8
           T+L+MALY
Sbjct: 140 TELQMALY 147
>NP_005496
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KLEMALYM 9
           KL+++LYM
Sbjct: 387 KLQLSLYM 394
>NP_001076428
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KLEMALYM 9
           KL+++LYM
Sbjct: 387 KLQLSLYM 394
>NP_689590
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLEMALYM 9
           KLE ALY+
Sbjct: 480 KLENALYL 487
>NP_001229831
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>NP_001229832
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>NP_003479
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_006722190
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_005257766
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_005257764
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_006722189
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TKLEMAL 7
           TKLE+AL
Sbjct: 129 TKLELAL 135
>NP_005992
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TKLEMALY 8
           +KLE A+Y
Sbjct: 165 SKLEFAIY 172
>NP_525125
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TKLEMALY 8
           +KLE A+Y
Sbjct: 165 SKLEFAIY 172
>NP_001180343
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TKLEMALY 8
           +KLE A+Y
Sbjct: 99  SKLEFAIY 106
>NP_112492
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LEMALYM 9
           LE+ALY+
Sbjct: 456 LELALYL 462
>NP_997195
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TKLEMALY 8
           +KLE A+Y
Sbjct: 165 SKLEFAIY 172
>NP_061816
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TKLEMALY 8
           +KLE A+Y
Sbjct: 165 SKLEFAIY 172
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.130    0.334 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,321
Number of extensions: 346
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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