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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055626                                                              18    1935
XP_006717405                                                           18    1935
XP_006717404                                                           18    1935
XP_006717403                                                           18    1935
XP_005263469                                                           18    1935
XP_005263468                                                           18    1935
NP_055876                                                              17    2528
XP_006716804                                                           17    2528
NP_001243355                                                           17    3301
XP_006714133                                                           17    3301
XP_005262753                                                           17    3301
XP_006714131                                                           17    3301
XP_005262752                                                           17    3301
XP_005262750                                                           17    3301
XP_006714130                                                           17    3301
XP_006714129                                                           17    3301
NP_525021                                                              15    9605

>NP_055626
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKARFSTA 9
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_006717405
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKARFSTA 9
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_006717404
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKARFSTA 9
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_006717403
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKARFSTA 9
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_005263469
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKARFSTA 9
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_005263468
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKARFSTA 9
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>NP_055876
          Length = 1056

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    KARFSTA 9
            KARFSTA
Sbjct: 995  KARFSTA 1001



>XP_006716804
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    KARFSTA 9
            KARFSTA
Sbjct: 1028 KARFSTA 1034



>NP_001243355
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 200 TKKAKFDSA 208



>XP_006714133
          Length = 605

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 180 TKKAKFDSA 188



>XP_005262753
          Length = 619

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 194 TKKAKFDSA 202



>XP_006714131
          Length = 630

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 205 TKKAKFDSA 213



>XP_005262752
          Length = 728

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 303 TKKAKFDSA 311



>XP_005262750
          Length = 734

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 309 TKKAKFDSA 317



>XP_006714130
          Length = 739

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 314 TKKAKFDSA 322



>XP_006714129
          Length = 739

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKKARFSTA 9
           TKKA+F +A
Sbjct: 314 TKKAKFDSA 322



>NP_525021
          Length = 1543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    TKKARFST 8
            TKK+ FST
Sbjct: 1208 TKKSCFST 1215



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.110    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 342,944
Number of extensions: 373
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)