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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245202                                                           20     509
NP_001245200                                                           20     509
NP_001161091                                                           20     509
NP_000240                                                              20     509
NP_001161090                                                           20     509
NP_001161089                                                           20     509
NP_001245203                                                           20     509
XP_005265223                                                           20     509
XP_005265221                                                           20     509
XP_005265220                                                           20     509
XP_005265218                                                           20     509
XP_005248231                                                           17    3301
NP_060793                                                              17    3301
NP_001073291                                                           16    5631
NP_000417                                                              16    5631
XP_005267039                                                           16    5631
XP_005267038                                                           16    5631
NP_001273135                                                           16    7355
NP_055710                                                              16    7355
NP_001704                                                              16    7355
XP_006715443                                                           16    7355
XP_005248732                                                           16    7355
XP_005248731                                                           16    7355
XP_005265877                                                           16    7355
NP_006179                                                              15    9605
NP_001091091                                                           15    9605

>NP_001245202
          Length = 515

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 211 TKGTSEMS 218



>NP_001245200
          Length = 687

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 452 TKGTSEMS 459



>NP_001161091
          Length = 515

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 211 TKGTSEMS 218



>NP_000240
          Length = 756

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 452 TKGTSEMS 459



>NP_001161090
          Length = 515

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 211 TKGTSEMS 218



>NP_001161089
          Length = 658

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 354 TKGTSEMS 361



>NP_001245203
          Length = 515

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 211 TKGTSEMS 218



>XP_005265223
          Length = 415

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 111 TKGTSEMS 118



>XP_005265221
          Length = 515

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 211 TKGTSEMS 218



>XP_005265220
          Length = 515

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 211 TKGTSEMS 218



>XP_005265218
          Length = 687

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TKGTSEMS 8
           TKGTSEMS
Sbjct: 383 TKGTSEMS 390



>XP_005248231
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  TKGTSEMSA 9
          T GTSEM+A
Sbjct: 91 TIGTSEMNA 99



>NP_060793
          Length = 481

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  TKGTSEMSA 9
          T GTSEM+A
Sbjct: 91 TIGTSEMNA 99



>NP_001073291
          Length = 3118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKGTSE 6
           TKGTSE
Sbjct: 797 TKGTSE 802



>NP_000417
          Length = 3122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKGTSE 6
           TKGTSE
Sbjct: 797 TKGTSE 802



>XP_005267039
          Length = 3206

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKGTSE 6
           TKGTSE
Sbjct: 797 TKGTSE 802



>XP_005267038
          Length = 3210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKGTSE 6
           TKGTSE
Sbjct: 797 TKGTSE 802



>NP_001273135
          Length = 1327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TKGTSEMSA 9
            +KG  EMSA
Sbjct: 1048 SKGREEMSA 1056



>NP_055710
          Length = 1403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TKGTSEMSA 9
            +KG  EMSA
Sbjct: 1124 SKGREEMSA 1132



>NP_001704
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TKGTSEM 7
           TKGT+E+
Sbjct: 376 TKGTAEL 382



>XP_006715443
          Length = 1327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TKGTSEMSA 9
            +KG  EMSA
Sbjct: 1048 SKGREEMSA 1056



>XP_005248732
          Length = 1330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TKGTSEMSA 9
            +KG  EMSA
Sbjct: 1051 SKGREEMSA 1059



>XP_005248731
          Length = 1365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TKGTSEMSA 9
            +KG  EMSA
Sbjct: 1086 SKGREEMSA 1094



>XP_005265877
          Length = 286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KGTSEMSA 9
          KG  EMSA
Sbjct: 8  KGVDEMSA 15



>NP_006179
          Length = 109

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TKGTSEMSA 9
          T G S+MSA
Sbjct: 33 TSGLSKMSA 41



>NP_001091091
          Length = 109

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TKGTSEMSA 9
          T G S+MSA
Sbjct: 33 TSGLSKMSA 41



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.282   0.0947    0.217 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 444,502
Number of extensions: 447
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)