Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     299
NP_872293                                                              17    3301
XP_005251817                                                           17    3301
XP_006717052                                                           17    3301
NP_699174                                                              17    4312
NP_542166                                                              16    5631
NP_056357                                                              16    5631
NP_689771                                                              16    5631
NP_001274429                                                           16    5631
NP_001274428                                                           16    5631
NP_006438                                                              16    7355
NP_001001992                                                           16    7355
NP_056323                                                              16    7355
NP_001027582                                                           16    7355
NP_078911                                                              16    7355
NP_001171646                                                           16    7355
NP_001171647                                                           16    7355
XP_005252185                                                           16    7355
XP_006717294                                                           16    7355
XP_005252184                                                           16    7355
XP_005252183                                                           16    7355
XP_006711313                                                           16    7355
NP_115813                                                              15    9605
NP_002678                                                              15    9605
NP_079276                                                              15    9605
NP_001158293                                                           15    9605
NP_036553                                                              15    9605
NP_001092286                                                           15    9605
NP_001269847                                                           15    9605
NP_065069                                                              15    9605
NP_001269846                                                           15    9605
XP_005260522                                                           15    9605
XP_005260521                                                           15    9605
XP_005260520                                                           15    9605
XP_005260519                                                           15    9605
XP_006718561                                                           15    9605
XP_005265906                                                           15    9605
XP_006714896                                                           15    9605
XP_006712835                                                           15    9605
XP_006712834                                                           15    9605
XP_006712833                                                           15    9605
XP_006712832                                                           15    9605
XP_006712831                                                           15    9605
XP_006712830                                                           15    9605
XP_006712829                                                           15    9605
XP_005271331                                                           15    9605
XP_006711040                                                           15    9605
XP_006711039                                                           15    9605
NP_079429                                                              15    9605
NP_001004690                                                           15    9605
>NP_004110
          Length = 993

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   KANRKVFG 9
           KANRKVFG
Sbjct: 486 KANRKVFG 493
>NP_872293
          Length = 652

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TKANRKVFG 9
          T+A+ KVFG
Sbjct: 25 TRADSKVFG 33
>XP_005251817
          Length = 569

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TKANRKVFG 9
          T+A+ KVFG
Sbjct: 25 TRADSKVFG 33
>XP_006717052
          Length = 616

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TKANRKVFG 9
          T+A+ KVFG
Sbjct: 25 TRADSKVFG 33
>NP_699174
          Length = 440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKANRKVF 8
           TKA RKV+
Sbjct: 128 TKAKRKVY 135
>NP_542166
          Length = 1272

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKANRK 6
           TKANRK
Sbjct: 597 TKANRK 602
>NP_056357
          Length = 1272

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TKANRK 6
           TKANRK
Sbjct: 597 TKANRK 602
>NP_689771
          Length = 695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  TKANRKVF 8
          TKA RK F
Sbjct: 28 TKAKRKAF 35
>NP_001274429
          Length = 686

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  TKANRKVF 8
          TKA RK F
Sbjct: 12 TKAKRKAF 19
>NP_001274428
          Length = 702

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  TKANRKVF 8
          TKA RK F
Sbjct: 28 TKAKRKAF 35
>NP_006438
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKANRKVF 8
           T ANR+VF
Sbjct: 619 TLANREVF 626
>NP_001001992
          Length = 822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKANRKVF 8
           T ANR+VF
Sbjct: 618 TLANREVF 625
>NP_056323
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NRK+FG
Sbjct: 515 NRKMFG 520
>NP_001027582
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TKANRKVF 8
           T ANR+VF
Sbjct: 619 TLANREVF 626
>NP_078911
          Length = 725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+KVFG
Sbjct: 379 NKKVFG 384
>NP_001171646
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NRK+FG
Sbjct: 515 NRKMFG 520
>NP_001171647
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NRK+FG
Sbjct: 515 NRKMFG 520
>XP_005252185
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+KVFG
Sbjct: 223 NKKVFG 228
>XP_006717294
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+KVFG
Sbjct: 379 NKKVFG 384
>XP_005252184
          Length = 725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+KVFG
Sbjct: 379 NKKVFG 384
>XP_005252183
          Length = 725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+KVFG
Sbjct: 379 NKKVFG 384
>XP_006711313
          Length = 942

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  NRKVFG 9
          NRK+FG
Sbjct: 20 NRKMFG 25
>NP_115813
          Length = 629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NR+VFG
Sbjct: 350 NREVFG 355
>NP_002678
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NR++FG
Sbjct: 142 NRRIFG 147
>NP_079276
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>NP_001158293
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>NP_036553
          Length = 1914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ANRKVFG 9
           +N KVFG
Sbjct: 928 SNEKVFG 934
>NP_001092286
          Length = 2769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    NRKVFG 9
            N+KVFG
Sbjct: 1125 NQKVFG 1130
>NP_001269847
          Length = 1491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 995  KANQKLF 1001
>NP_065069
          Length = 1494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 999  KANQKLF 1005
>NP_001269846
          Length = 1494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 999  KANQKLF 1005
>XP_005260522
          Length = 1495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 999  KANQKLF 1005
>XP_005260521
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 1007 KANQKLF 1013
>XP_005260520
          Length = 1506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 1011 KANQKLF 1017
>XP_005260519
          Length = 1507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KANRKVF 8
            KAN+K+F
Sbjct: 1011 KANQKLF 1017
>XP_006718561
          Length = 2794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    NRKVFG 9
            N+KVFG
Sbjct: 1150 NQKVFG 1155
>XP_005265906
          Length = 1421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KANRKVFG 9
           K +RK+FG
Sbjct: 928 KPDRKLFG 935
>XP_006714896
          Length = 1461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KANRKVFG 9
           K +RK+FG
Sbjct: 968 KPDRKLFG 975
>XP_006712835
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>XP_006712834
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>XP_006712833
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>XP_006712832
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>XP_006712831
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 120 NKKIFG 125
>XP_006712830
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 120 NKKIFG 125
>XP_006712829
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           N+K+FG
Sbjct: 207 NKKIFG 212
>XP_005271331
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NR+VFG
Sbjct: 350 NREVFG 355
>XP_006711040
          Length = 629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NR+VFG
Sbjct: 350 NREVFG 355
>XP_006711039
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NRKVFG 9
           NR+VFG
Sbjct: 363 NREVFG 368
>NP_079429
          Length = 1461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KANRKVFG 9
           K +RK+FG
Sbjct: 968 KPDRKLFG 975
>NP_001004690
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TKANRKVFG 9
           T+A RKV G
Sbjct: 298 TRALRKVLG 306
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.132    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 373,051
Number of extensions: 437
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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