Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     509
NP_001035957                                                           20     509
XP_006721991                                                           20     509
XP_006721990                                                           20     509
XP_006721989                                                           20     509
XP_005258041                                                           20     509
XP_006721988                                                           20     509
XP_006721987                                                           20     509
XP_006721986                                                           20     509
XP_005258040                                                           20     509
XP_006721985                                                           20     509
NP_006840                                                              17    4312
NP_001277114                                                           16    5631
NP_068375                                                              16    5631
NP_002431                                                              16    7355
NP_001153239                                                           16    7355
NP_002106                                                              16    7355
NP_054757                                                              16    7355
NP_056052                                                              16    7355
NP_001074003                                                           16    7355
XP_005254452                                                           16    7355
XP_005254451                                                           16    7355
XP_006715771                                                           16    7355
XP_006715770                                                           16    7355
XP_006715769                                                           16    7355
NP_777557                                                              16    7355
NP_000431                                                              15    9605
NP_006436                                                              15    9605
NP_569733                                                              15    9605
NP_904324                                                              15    9605
NP_775922                                                              15    9605
XP_006719745                                                           15    9605
XP_006719744                                                           15    9605
XP_005254044                                                           15    9605
NP_061720                                                              15    9605
>NP_000258
          Length = 2818

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 940 TISKFFDSQ 948
>NP_001035957
          Length = 2839

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 940 TISKFFDSQ 948
>XP_006721991
          Length = 1632

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 950 TISKFFDSQ 958
>XP_006721990
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 950 TISKFFDSQ 958
>XP_006721989
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 950 TISKFFDSQ 958
>XP_005258041
          Length = 2836

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 940 TISKFFDSQ 948
>XP_006721988
          Length = 2846

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 950 TISKFFDSQ 958
>XP_006721987
          Length = 2849

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 950 TISKFFDSQ 958
>XP_006721986
          Length = 2854

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 937 TISKFFDSQ 945
>XP_005258040
          Length = 2857

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 940 TISKFFDSQ 948
>XP_006721985
          Length = 2867

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TISKFLDSQ 9
           TISKF DSQ
Sbjct: 950 TISKFFDSQ 958
>NP_006840
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TISKFLDS 8
           T SKFLD+
Sbjct: 486 TFSKFLDN 493
>NP_001277114
          Length = 931

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISKFLDSQ 9
           I KFLD+Q
Sbjct: 414 IRKFLDAQ 421
>NP_068375
          Length = 941

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISKFLDSQ 9
           I KFLD+Q
Sbjct: 424 IRKFLDAQ 431
>NP_002431
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ISKFLDSQ 9
           IS+FLD++
Sbjct: 389 ISRFLDTE 396
>NP_001153239
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ISKFLDS 8
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>NP_002106
          Length = 923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ISKFLDSQ 9
           +S+FLD+Q
Sbjct: 147 LSEFLDAQ 154
>NP_054757
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ISKFLDS 8
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>NP_056052
          Length = 1354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKFLDS 8
            SKFLDS
Sbjct: 1132 SKFLDS 1137
>NP_001074003
          Length = 2214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ISKFLDSQ 9
            I KF+D+Q
Sbjct: 2025 IKKFIDTQ 2032
>XP_005254452
          Length = 2188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ISKFLDSQ 9
            I KF+D+Q
Sbjct: 1999 IKKFIDTQ 2006
>XP_005254451
          Length = 2214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ISKFLDSQ 9
            I KF+D+Q
Sbjct: 2025 IKKFIDTQ 2032
>XP_006715771
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ISKFLDS 8
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>XP_006715770
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ISKFLDS 8
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>XP_006715769
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ISKFLDS 8
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>NP_777557
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISKFLDS 8
           ++KFLDS
Sbjct: 157 VNKFLDS 163
>NP_000431
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  ISKFLDS 8
          + KFLDS
Sbjct: 9  VEKFLDS 15
>NP_006436
          Length = 2335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SKFLDSQ 9
            S+F+DSQ
Sbjct: 1377 SEFIDSQ 1383
>NP_569733
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TISKFLDS 8
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>NP_904324
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TISKFLDS 8
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>NP_775922
          Length = 6996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    TISKFLDS 8
            T+SK +DS
Sbjct: 4131 TVSKIVDS 4138
>XP_006719745
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TISKFLDS 8
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>XP_006719744
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TISKFLDS 8
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>XP_005254044
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TISKFLDS 8
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>NP_061720
          Length = 4024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TISKFLD 7
           T+S FLD
Sbjct: 872 TVSSFLD 878
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.127    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 440,925
Number of extensions: 735
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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