Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     869
NP_065852                                                              18    1935
NP_004546                                                              17    2528
NP_775186                                                              17    2528
NP_775188                                                              17    2528
NP_060697                                                              17    4312
NP_060557                                                              17    4312
XP_006714316                                                           17    4312
XP_006710825                                                           17    4312
NP_001162023                                                           17    4312
NP_065788                                                              16    5631
NP_057341                                                              16    5631
NP_001269325                                                           16    5631
NP_001269326                                                           16    5631
NP_005804                                                              16    7355
NP_570843                                                              16    7355
NP_001128529                                                           16    7355
NP_001073351                                                           16    7355
NP_001073350                                                           16    7355
NP_001073349                                                           16    7355
NP_057541                                                              16    7355
XP_005258773                                                           16    7355
XP_006712043                                                           16    7355
XP_006712042                                                           16    7355
XP_006712041                                                           16    7355
XP_005264295                                                           16    7355
XP_005264294                                                           16    7355
NP_775766                                                              15    9605
NP_002733                                                              15    9605
NP_001239049                                                           15    9605
NP_848663                                                              15    9605
NP_055963                                                              15    9605
NP_001273700                                                           15    9605
NP_115593                                                              15    9605
NP_942592                                                              15    9605
NP_001239053                                                           15    9605
NP_001239051                                                           15    9605
NP_001239048                                                           15    9605
NP_001073920                                                           15    9605
XP_006722001                                                           15    9605
XP_006722000                                                           15    9605
XP_006721999                                                           15    9605
XP_006721998                                                           15    9605
XP_006721997                                                           15    9605
XP_006721996                                                           15    9605
XP_006721995                                                           15    9605
XP_005267916                                                           15    9605
XP_006720168                                                           15    9605
XP_005265728                                                           15    9605
XP_005265724                                                           15    9605
XP_005247048                                                           15    9605
XP_005247047                                                           15    9605
NP_057423                                                              15    9605
>NP_002430
          Length = 1137

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   ISGIVNLE 9
           ISGIVNLE
Sbjct: 489 ISGIVNLE 496
>NP_065852
          Length = 414

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  TISGIVNLE 9
          T+SG VNLE
Sbjct: 29 TVSGRVNLE 37
>NP_004546
          Length = 1068

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TISGIVNL 8
           T+SG+VNL
Sbjct: 837 TLSGLVNL 844
>NP_775186
          Length = 1065

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TISGIVNL 8
           T+SG+VNL
Sbjct: 837 TLSGLVNL 844
>NP_775188
          Length = 1075

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TISGIVNL 8
           T+SG+VNL
Sbjct: 837 TLSGLVNL 844
>NP_060697
          Length = 1052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TISGIVNLE 9
           T+SG+V LE
Sbjct: 900 TVSGLVALE 908
>NP_060557
          Length = 674

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TISGIVNL 8
           T+SG++NL
Sbjct: 260 TVSGLLNL 267
>XP_006714316
          Length = 977

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TISGIVNLE 9
           T+SG+V LE
Sbjct: 825 TVSGLVALE 833
>XP_006710825
          Length = 669

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TISGIVNL 8
           T+SG++NL
Sbjct: 255 TVSGLLNL 262
>NP_001162023
          Length = 634

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TISGIVNL 8
           T+SG++NL
Sbjct: 220 TVSGLLNL 227
>NP_065788
          Length = 789

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TISGIVNLE 9
           T+ G+VNL+
Sbjct: 119 TLRGLVNLQ 127
>NP_057341
          Length = 191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SGIVNLE 9
          SGI+NLE
Sbjct: 40 SGILNLE 46
>NP_001269325
          Length = 191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SGIVNLE 9
          SGI+NLE
Sbjct: 40 SGILNLE 46
>NP_001269326
          Length = 119

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SGIVNLE 9
          SGI+NLE
Sbjct: 40 SGILNLE 46
>NP_005804
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 634 GIVNLE 639
>NP_570843
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TISGIVNLE 9
           T  G+VNL+
Sbjct: 216 TFDGLVNLQ 224
>NP_001128529
          Length = 587

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TISGIVNLE 9
           T  G+VNL+
Sbjct: 222 TFDGLVNLQ 230
>NP_001073351
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 452 GIVNLE 457
>NP_001073350
          Length = 878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 609 GIVNLE 614
>NP_001073349
          Length = 878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 609 GIVNLE 614
>NP_057541
          Length = 878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 609 GIVNLE 614
>XP_005258773
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 452 GIVNLE 457
>XP_006712043
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 375 GIVNLE 380
>XP_006712042
          Length = 717

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 461 GIVNLE 466
>XP_006712041
          Length = 717

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 461 GIVNLE 466
>XP_005264295
          Length = 786

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 530 GIVNLE 535
>XP_005264294
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           GIVNLE
Sbjct: 634 GIVNLE 639
>NP_775766
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           T +GI+NL
Sbjct: 313 TAAGIINL 320
>NP_002733
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           G+VNLE
Sbjct: 641 GVVNLE 646
>NP_001239049
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 344 TISGLITL 351
>NP_848663
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TISGIVNLE 9
           T  G+VNL+
Sbjct: 192 TFRGLVNLD 200
>NP_055963
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 344 TISGLITL 351
>NP_001273700
          Length = 2490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SGIVNLE 9
            SGIVN++
Sbjct: 2207 SGIVNMD 2213
>NP_115593
          Length = 2603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SGIVNLE 9
            SGIVN++
Sbjct: 2320 SGIVNMD 2326
>NP_942592
          Length = 2352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SGIVNLE 9
            SGIVN++
Sbjct: 2069 SGIVNMD 2075
>NP_001239053
          Length = 1143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 344 TISGLITL 351
>NP_001239051
          Length = 1288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 309 TISGLITL 316
>NP_001239048
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 344 TISGLITL 351
>NP_001073920
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TISGIVNLE 9
           +ISG  NLE
Sbjct: 316 SISGTYNLE 324
>XP_006722001
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 324 VDGLVNIE 331
>XP_006722000
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 388 VDGLVNIE 395
>XP_006721999
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 425 VDGLVNIE 432
>XP_006721998
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 425 VDGLVNIE 432
>XP_006721997
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 425 VDGLVNIE 432
>XP_006721996
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 443 VDGLVNIE 450
>XP_006721995
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 594 VDGLVNIE 601
>XP_005267916
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GIVNLE 9
           G+VNLE
Sbjct: 649 GVVNLE 654
>XP_006720168
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TISGIVNLE 9
           +ISG  NLE
Sbjct: 112 SISGTYNLE 120
>XP_005265728
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SGIVNLE 9
            SGIVN++
Sbjct: 2068 SGIVNMD 2074
>XP_005265724
          Length = 2602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SGIVNLE 9
            SGIVN++
Sbjct: 2319 SGIVNMD 2325
>XP_005247048
          Length = 1107

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 128 TISGLITL 135
>XP_005247047
          Length = 1137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TISGIVNL 8
           TISG++ L
Sbjct: 158 TISGLITL 165
>NP_057423
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ISGIVNLE 9
           + G+VN+E
Sbjct: 388 VDGLVNIE 395
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.136    0.338 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 431,684
Number of extensions: 682
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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