Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     869
XP_005262424                                                           19     869
NP_689777                                                              17    3301
NP_060360                                                              17    3301
NP_056058                                                              17    3301
XP_006716574                                                           17    3301
XP_006716573                                                           17    3301
XP_005250858                                                           17    3301
XP_005250857                                                           17    3301
NP_001070710                                                           16    7355
NP_001714                                                              15    9605
NP_443075                                                              15    9605
NP_001258152                                                           15    9605
NP_001092093                                                           15    9605
NP_149438                                                              15    9605
NP_001014765                                                           15    9605
XP_005249383                                                           15    9605
XP_006711892                                                           15    9605
XP_006711891                                                           15    9605
XP_006711890                                                           15    9605
XP_006711889                                                           15    9605
XP_005273367                                                           15    9605
XP_005273366                                                           15    9605
XP_006711888                                                           15    9605
XP_006711887                                                           15    9605
XP_005273364                                                           15    9605
XP_005273362                                                           15    9605
XP_005273361                                                           15    9605
XP_006711886                                                           15    9605
XP_006711885                                                           15    9605
XP_005273359                                                           15    9605
XP_006711884                                                           15    9605
XP_005273357                                                           15    9605
XP_005273356                                                           15    9605
XP_005273358                                                           15    9605
XP_005273355                                                           15    9605
XP_005273353                                                           15    9605
XP_005273352                                                           15    9605
XP_005273354                                                           15    9605
XP_005273349                                                           15    9605
XP_006711883                                                           15    9605
XP_005273350                                                           15    9605
XP_005273351                                                           15    9605
XP_006711882                                                           15    9605
XP_005273348                                                           15    9605
XP_005273346                                                           15    9605
XP_005273347                                                           15    9605
XP_005273345                                                           15    9605
XP_005273344                                                           15    9605
XP_006711109                                                           15    9605
XP_006711108                                                           15    9605
XP_005263595                                                           15    9605
XP_006711107                                                           15    9605
>NP_722576
          Length = 3080

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TIQEKSTAV 9
           TIQEKST V
Sbjct: 235 TIQEKSTTV 243
>XP_005262424
          Length = 2875

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TIQEKSTAV 9
          TIQEKST V
Sbjct: 30 TIQEKSTTV 38
>NP_689777
          Length = 3997

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>NP_060360
          Length = 4022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>NP_056058
          Length = 863

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_006716574
          Length = 968

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_006716573
          Length = 1776

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_005250858
          Length = 4022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>XP_005250857
          Length = 4022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TIQEKST 7
           T+QEKST
Sbjct: 568 TVQEKST 574
>NP_001070710
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  TIQEKSTAV 9
          T+QEK+ A+
Sbjct: 3  TVQEKAAAL 11
>NP_001714
          Length = 2649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IQEKSTA 8
            IQ+KSTA
Sbjct: 1867 IQKKSTA 1873
>NP_443075
          Length = 6620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 2927 VEEKGTAV 2934
>NP_001258152
          Length = 8923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3356 VEEKGTAV 3363
>NP_001092093
          Length = 7968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 2927 VEEKGTAV 2934
>NP_149438
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TIQEKSTA 8
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>NP_001014765
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TIQEKSTA 8
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_005249383
          Length = 3186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    IQEKSTA 8
            IQ+KSTA
Sbjct: 2404 IQKKSTA 2410
>XP_006711892
          Length = 6865

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3172 VEEKGTAV 3179
>XP_006711891
          Length = 8925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3356 VEEKGTAV 3363
>XP_006711890
          Length = 6957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711889
          Length = 7297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273367
          Length = 7677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273366
          Length = 7678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711888
          Length = 8059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711887
          Length = 8499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273364
          Length = 8653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273362
          Length = 8675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273361
          Length = 8763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711886
          Length = 8833

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711885
          Length = 8835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273359
          Length = 8843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3080 VEEKGTAV 3087
>XP_006711884
          Length = 8847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273357
          Length = 8851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273356
          Length = 8851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273358
          Length = 8851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273355
          Length = 8924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273353
          Length = 8935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3172 VEEKGTAV 3179
>XP_005273352
          Length = 8935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3172 VEEKGTAV 3179
>XP_005273354
          Length = 8935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3172 VEEKGTAV 3179
>XP_005273349
          Length = 8939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711883
          Length = 8939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273350
          Length = 8939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273351
          Length = 8939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711882
          Length = 8977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3214 VEEKGTAV 3221
>XP_005273348
          Length = 9015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273346
          Length = 9026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273347
          Length = 9026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273345
          Length = 9026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_005273344
          Length = 9027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IQEKSTAV 9
            ++EK TAV
Sbjct: 3264 VEEKGTAV 3271
>XP_006711109
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TIQEKSTA 8
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_006711108
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TIQEKSTA 8
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_005263595
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TIQEKSTA 8
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
>XP_006711107
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TIQEKSTA 8
           TIQ+KS A
Sbjct: 197 TIQQKSDA 204
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294    0.104    0.228 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 427,881
Number of extensions: 208
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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