Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_065949                                                              17    2528
XP_006719586                                                           17    2528
NP_689472                                                              17    3301
NP_001186208                                                           17    4312
NP_004189                                                              17    4312
NP_003748                                                              17    4312
NP_006065                                                              16    5631
NP_001186502                                                           16    5631
XP_005250861                                                           16    7355
XP_005248910                                                           16    7355
XP_005248908                                                           16    7355
XP_006715060                                                           16    7355
XP_005248909                                                           16    7355
XP_005248907                                                           16    7355
NP_001139124                                                           16    7355
NP_620172                                                              16    7355
NP_001186491                                                           15    9605
NP_003551                                                              15    9605
NP_001004426                                                           15    9605
NP_001180214                                                           15    9605
NP_057623                                                              15    9605
NP_003782                                                              15    9605
NP_079265                                                              15    9605
NP_116279                                                              15    9605
NP_001035100                                                           15    9605
NP_004902                                                              15    9605
XP_006724395                                                           15    9605
XP_005261823                                                           15    9605
XP_005261822                                                           15    9605
XP_005261821                                                           15    9605
XP_005259634                                                           15    9605
XP_005259632                                                           15    9605
XP_006722830                                                           15    9605
XP_005257805                                                           15    9605
XP_006722205                                                           15    9605
XP_006722204                                                           15    9605
XP_006721061                                                           15    9605
XP_005267641                                                           15    9605
XP_005252039                                                           15    9605
XP_006716248                                                           15    9605
XP_005246923                                                           15    9605
XP_006711658                                                           15    9605
XP_005273393                                                           15    9605
XP_006711657                                                           15    9605
XP_006711656                                                           15    9605
XP_005273392                                                           15    9605
XP_006711655                                                           15    9605
XP_006710064                                                           15    9605
NP_003623                                                              15    9605
NP_001012993                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TFRSSRPMD 9
           TFRSSR MD
Sbjct: 137 TFRSSRLMD 145
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TFRSSRPMD 9
           TFRSSR MD
Sbjct: 137 TFRSSRLMD 145
>NP_065949
          Length = 691

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR  RPMD
Sbjct: 118 FREPRPMD 125
>XP_006719586
          Length = 657

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR  RPMD
Sbjct: 118 FREPRPMD 125
>NP_689472
          Length = 1910

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TFRSSRPMD 9
           T RSS+P D
Sbjct: 626 TLRSSKPKD 634
>NP_001186208
          Length = 479

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FRSSRPMD 9
           F+SS PMD
Sbjct: 139 FKSSCPMD 146
>NP_004189
          Length = 494

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FRSSRPMD 9
           F+SS PMD
Sbjct: 154 FKSSCPMD 161
>NP_003748
          Length = 325

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TFRSSRPMD 9
           TFR+ RP++
Sbjct: 225 TFRTERPVN 233
>NP_006065
          Length = 498

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TFRSSRPMD 9
           TF++S P D
Sbjct: 330 TFKNSNPCD 338
>NP_001186502
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TFRSSRPMD 9
           TF++S P D
Sbjct: 326 TFKNSNPCD 334
>XP_005250861
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FRSSRPM 8
           F+SSRP+
Sbjct: 299 FKSSRPI 305
>XP_005248910
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TFRSSR 6
           TFRSSR
Sbjct: 194 TFRSSR 199
>XP_005248908
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TFRSSR 6
           TFRSSR
Sbjct: 194 TFRSSR 199
>XP_006715060
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TFRSSR 6
           TFRSSR
Sbjct: 194 TFRSSR 199
>XP_005248909
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TFRSSR 6
           TFRSSR
Sbjct: 194 TFRSSR 199
>XP_005248907
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TFRSSR 6
           TFRSSR
Sbjct: 194 TFRSSR 199
>NP_001139124
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TFRSSR 6
           TFRSSR
Sbjct: 194 TFRSSR 199
>NP_620172
          Length = 470

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FRSSRPM 8
           F+SSRP+
Sbjct: 308 FKSSRPI 314
>NP_001186491
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 495 FRGSRPYE 502
>NP_003551
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 549 FRGSRPYE 556
>NP_001004426
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 495 FRGSRPYE 502
>NP_001180214
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TFRSSRP 7
          TF S+RP
Sbjct: 85 TFESARP 91
>NP_057623
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TFRSSRP 7
          TF S+RP
Sbjct: 85 TFESARP 91
>NP_003782
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FRSSRP 7
           FRS+RP
Sbjct: 965 FRSNRP 970
>NP_079265
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TFRSSRPMD 9
           TF++S+P++
Sbjct: 772 TFKNSQPVN 780
>NP_116279
          Length = 1524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 755 FRSSHPFE 762
>NP_001035100
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRSSRPM 8
           FRS RP+
Sbjct: 139 FRSPRPL 145
>NP_004902
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   FRSSRPMD 9
           FR  RP D
Sbjct: 258 FRKGRPYD 265
>XP_006724395
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 549 FRGSRPYE 556
>XP_005261823
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 549 FRGSRPYE 556
>XP_005261822
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 549 FRGSRPYE 556
>XP_005261821
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FR SRP +
Sbjct: 549 FRGSRPYE 556
>XP_005259634
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TFRSSRP 7
          TF S+RP
Sbjct: 85 TFESARP 91
>XP_005259632
          Length = 283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TFRSSRP 7
          TF S+RP
Sbjct: 13 TFESARP 19
>XP_006722830
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TFRSSRP 7
          TF S+RP
Sbjct: 61 TFESARP 67
>XP_005257805
          Length = 1308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FRSSRPM 8
            FR+ RPM
Sbjct: 1149 FRTPRPM 1155
>XP_006722205
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FRSSRPM 8
            FR+ RPM
Sbjct: 1225 FRTPRPM 1231
>XP_006722204
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FRSSRPM 8
            FR+ RPM
Sbjct: 1225 FRTPRPM 1231
>XP_006721061
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  SSRPMD 9
          SSRP+D
Sbjct: 6  SSRPLD 11
>XP_005267641
          Length = 244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RSSRPMD 9
           +SSRP D
Sbjct: 196 KSSRPFD 202
>XP_005252039
          Length = 139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  FRSSRPM 8
          FRS RP+
Sbjct: 43 FRSPRPL 49
>XP_006716248
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   FRSSRPMD 9
           FR  RP D
Sbjct: 258 FRKGRPYD 265
>XP_005246923
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TFRSSRPMD 9
           TF++S+P++
Sbjct: 598 TFKNSQPVN 606
>XP_006711658
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 466 FRSSHPFE 473
>XP_005273393
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 561 FRSSHPFE 568
>XP_006711657
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 561 FRSSHPFE 568
>XP_006711656
          Length = 1524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 755 FRSSHPFE 762
>XP_005273392
          Length = 1524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 755 FRSSHPFE 762
>XP_006711655
          Length = 1524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FRSSRPMD 9
           FRSS P +
Sbjct: 755 FRSSHPFE 762
>XP_006710064
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  SSRPMD 9
          SSRP+D
Sbjct: 6  SSRPLD 11
>NP_003623
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FRSSRPM 8
            FR+ RPM
Sbjct: 1225 FRTPRPM 1231
>NP_001012993
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RSSRPMD 9
           +SSRP D
Sbjct: 168 KSSRPFD 174
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.128    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 623,032
Number of extensions: 952
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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