Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_055309                                                              17    2528
XP_006721103                                                           17    2528
XP_005255314                                                           17    2528
XP_006721102                                                           17    2528
XP_005255313                                                           17    2528
XP_005255312                                                           17    2528
XP_006721101                                                           17    2528
XP_005255311                                                           17    2528
XP_005255310                                                           17    2528
NP_055839                                                              17    3301
NP_000098                                                              17    4312
XP_006718224                                                           17    4312
NP_056417                                                              16    5631
NP_001254507                                                           16    5631
NP_055426                                                              16    7355
NP_056993                                                              16    7355
XP_005248757                                                           16    7355
XP_006715468                                                           16    7355
XP_005248756                                                           16    7355
XP_006715467                                                           16    7355
NP_002215                                                              15    9605
NP_056034                                                              15    9605
NP_001269243                                                           15    9605
NP_001269242                                                           15    9605
NP_001013861                                                           15    9605
NP_001138771                                                           15    9605
NP_001138770                                                           15    9605
NP_001138769                                                           15    9605
XP_006721852                                                           15    9605
XP_006721851                                                           15    9605
XP_006721850                                                           15    9605
XP_006721849                                                           15    9605
XP_006715155                                                           15    9605
XP_006715154                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ESTTRLQE 9
            ESTTRLQE
Sbjct: 2239 ESTTRLQE 2246
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ESTTRLQE 9
            ESTTRLQE
Sbjct: 2231 ESTTRLQE 2238
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ESTTRLQE 9
            ESTTRLQE
Sbjct: 2192 ESTTRLQE 2199
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ESTTRLQE 9
            ESTTRLQE
Sbjct: 2271 ESTTRLQE 2278
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ESTTRLQE 9
            ESTTRLQE
Sbjct: 2284 ESTTRLQE 2291
>NP_055309
          Length = 1962

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 676 TESTGRLEE 684
>XP_006721103
          Length = 1597

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 644 TESTGRLEE 652
>XP_005255314
          Length = 1709

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 423 TESTGRLEE 431
>XP_006721102
          Length = 1820

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 534 TESTGRLEE 542
>XP_005255313
          Length = 1822

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 644 TESTGRLEE 652
>XP_005255312
          Length = 1871

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 644 TESTGRLEE 652
>XP_006721101
          Length = 1907

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 644 TESTGRLEE 652
>XP_005255311
          Length = 1913

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 676 TESTGRLEE 684
>XP_005255310
          Length = 1919

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TESTTRLQE 9
           TEST RL+E
Sbjct: 644 TESTGRLEE 652
>NP_055839
          Length = 1087

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  TESTTRLQ 8
          T++TTRLQ
Sbjct: 22 TDTTTRLQ 29
>NP_000098
          Length = 427

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ESTTRLQE 9
           E TTRLQ+
Sbjct: 178 EGTTRLQD 185
>XP_006718224
          Length = 427

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ESTTRLQE 9
           E TTRLQ+
Sbjct: 178 EGTTRLQD 185
>NP_056417
          Length = 583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TESTTRLQE 9
           T  TTRLQ+
Sbjct: 124 TRRTTRLQQ 132
>NP_001254507
          Length = 584

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TESTTRLQE 9
           T  TTRLQ+
Sbjct: 124 TRRTTRLQQ 132
>NP_055426
          Length = 5596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 4382 QSTTRLTE 4389
>NP_056993
          Length = 2371

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TESTTRLQE 9
            TE+TT+L +
Sbjct: 1027 TEATTKLHD 1035
>XP_005248757
          Length = 3591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 2377 QSTTRLTE 2384
>XP_006715468
          Length = 3744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 2530 QSTTRLTE 2537
>XP_005248756
          Length = 3905

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 2691 QSTTRLTE 2698
>XP_006715467
          Length = 5550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 4382 QSTTRLTE 4389
>NP_002215
          Length = 2671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 1503 QSTTRLLE 1510
>NP_056034
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>NP_001269243
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>NP_001269242
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 26 TENLQRLQE 34
>NP_001013861
          Length = 684

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>NP_001138771
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>NP_001138770
          Length = 676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>NP_001138769
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>XP_006721852
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>XP_006721851
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>XP_006721850
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>XP_006721849
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  TESTTRLQE 9
          TE+  RLQE
Sbjct: 67 TENLQRLQE 75
>XP_006715155
          Length = 1850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 1532 QSTTRLLE 1539
>XP_006715154
          Length = 2700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ESTTRLQE 9
            +STTRL E
Sbjct: 1532 QSTTRLLE 1539
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288    0.101    0.228 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 436,911
Number of extensions: 677
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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