Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_001273897                                                           16    7355
NP_005345                                                              16    7355
NP_055704                                                              16    7355
NP_001229236                                                           16    7355
NP_001229238                                                           16    7355
XP_006717425                                                           16    7355
XP_005252402                                                           16    7355
NP_000362                                                              15    9605
NP_001389                                                              15    9605
XP_005258667                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TELAFTASD 9
           TELA TASD
Sbjct: 915 TELALTASD 923
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TELAFTASD 9
           TELA TASD
Sbjct: 915 TELALTASD 923
>NP_001273897
          Length = 304

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TELAFTAS 8
           TELA TAS
Sbjct: 265 TELASTAS 272
>NP_005345
          Length = 347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TELAFTAS 8
           TELA TAS
Sbjct: 308 TELASTAS 315
>NP_055704
          Length = 1037

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AFTASD 9
           AFTASD
Sbjct: 137 AFTASD 142
>NP_001229236
          Length = 1038

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AFTASD 9
           AFTASD
Sbjct: 137 AFTASD 142
>NP_001229238
          Length = 939

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AFTASD 9
           AFTASD
Sbjct: 105 AFTASD 110
>XP_006717425
          Length = 867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AFTASD 9
           AFTASD
Sbjct: 137 AFTASD 142
>XP_005252402
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AFTASD 9
           AFTASD
Sbjct: 137 AFTASD 142
>NP_000362
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ELAFTASD 9
           E+ FTA+D
Sbjct: 112 EVVFTAND 119
>NP_001389
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ELAFTA 7
           ELAFTA
Sbjct: 224 ELAFTA 229
>XP_005258667
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  ELAFTA 7
          ELAFTA
Sbjct: 93 ELAFTA 98
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.115    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 299,594
Number of extensions: 231
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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