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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_000065                                                              16    5631
XP_006720320                                                           16    5631
XP_005268130                                                           16    5631
NP_997545                                                              16    5631
NP_001191827                                                           16    7355
NP_001191826                                                           16    7355
NP_612470                                                              16    7355
NP_001098099                                                           16    7355
NP_060772                                                              16    7355

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    EASSRTTI 9
            EASSRTTI
Sbjct: 2106 EASSRTTI 2113



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    EASSRTTI 9
            EASSRTTI
Sbjct: 1901 EASSRTTI 1908



>NP_000065
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TEASSRTT 8
           +EASS+TT
Sbjct: 128 SEASSKTT 135



>XP_006720320
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TEASSRTT 8
           TE+ SRTT
Sbjct: 108 TESQSRTT 115



>XP_005268130
          Length = 438

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TEASSRTT 8
           TE+ SRTT
Sbjct: 108 TESQSRTT 115



>NP_997545
          Length = 306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TEASSRTT 8
           TE+ SRTT
Sbjct: 108 TESQSRTT 115



>NP_001191827
          Length = 183

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  TEASSRT 7
          T+ASSRT
Sbjct: 7  TQASSRT 13



>NP_001191826
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  TEASSRT 7
          T+ASSRT
Sbjct: 7  TQASSRT 13



>NP_612470
          Length = 209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  TEASSRT 7
          T+ASSRT
Sbjct: 7  TQASSRT 13



>NP_001098099
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  TEASSRT 7
          TE+SSRT
Sbjct: 64 TESSSRT 70



>NP_060772
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  TEASSRT 7
          TE+SSRT
Sbjct: 64 TESSSRT 70



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290   0.0972    0.213 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 224,159
Number of extensions: 253
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)