Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              19     869
XP_006715292                                                           19     869
NP_004045                                                              16    5631
XP_005248043                                                           16    5631
XP_006713958                                                           16    5631
NP_065824                                                              16    5631
NP_001106832                                                           16    5631
NP_001129074                                                           16    7355
NP_001073279                                                           16    7355
NP_000395                                                              16    7355
NP_055616                                                              16    7355
NP_003250                                                              16    7355
XP_005259159                                                           16    7355
XP_006711879                                                           16    7355
XP_005273329                                                           16    7355
NP_065913                                                              16    7355
NP_787050                                                              15    9605
NP_940820                                                              15    9605
NP_001028776                                                           15    9605
NP_001028777                                                           15    9605
NP_055266                                                              15    9605
NP_443078                                                              15    9605
NP_056381                                                              15    9605
XP_006721993                                                           15    9605
XP_006719863                                                           15    9605
XP_005266390                                                           15    9605
XP_006719862                                                           15    9605
XP_005266389                                                           15    9605
XP_005249758                                                           15    9605
XP_006715751                                                           15    9605
XP_005249757                                                           15    9605
XP_006715750                                                           15    9605
XP_005248537                                                           15    9605
XP_005248536                                                           15    9605
NP_057085                                                              15    9605
>NP_115927
          Length = 1321

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TDA LSTST
Sbjct: 315 TDALLSTST 323
>XP_006715292
          Length = 759

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TDA LSTST
Sbjct: 302 TDALLSTST 310
>NP_004045
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TDAFLST 7
           +DAFLST
Sbjct: 286 SDAFLST 292
>XP_005248043
          Length = 669

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  TDAFLSTST 9
          TD  LSTST
Sbjct: 2  TDGKLSTST 10
>XP_006713958
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  TDAFLSTST 9
          TD  LSTST
Sbjct: 2  TDGKLSTST 10
>NP_065824
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  TDAFLSTST 9
          TD  LSTST
Sbjct: 2  TDGKLSTST 10
>NP_001106832
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  TDAFLSTST 9
          TD  LSTST
Sbjct: 2  TDGKLSTST 10
>NP_001129074
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TDAFLS 6
           TDAFLS
Sbjct: 118 TDAFLS 123
>NP_001073279
          Length = 647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TDAFLS 6
           TDAFLS
Sbjct: 219 TDAFLS 224
>NP_000395
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TDAFLS 6
           TDAFLS
Sbjct: 249 TDAFLS 254
>NP_055616
          Length = 2137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TDAFLSTST 9
           TD FLS S+
Sbjct: 537 TDVFLSKSS 545
>NP_003250
          Length = 924

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   DAFLSTST 9
           DAF++T+T
Sbjct: 280 DAFVATAT 287
>XP_005259159
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DAFLST 7
           DAFLST
Sbjct: 158 DAFLST 163
>XP_006711879
          Length = 1364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TDAFLSTST 9
           TD FLS S+
Sbjct: 537 TDVFLSKSS 545
>XP_005273329
          Length = 1934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TDAFLSTST 9
           TD FLS S+
Sbjct: 334 TDVFLSKSS 342
>NP_065913
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DAFLST 7
           DAFLST
Sbjct: 158 DAFLST 163
>NP_787050
          Length = 887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TDAFLSTS 8
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>NP_940820
          Length = 887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>NP_001028776
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>NP_001028777
          Length = 882

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>NP_055266
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>NP_443078
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           +DAFL+ +T
Sbjct: 609 SDAFLAANT 617
>NP_056381
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   DAFLSTS 8
           DAF STS
Sbjct: 310 DAFASTS 316
>XP_006721993
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TDAFLSTST 9
           T  F+STST
Sbjct: 238 TGNFISTST 246
>XP_006719863
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TDAFLSTS 8
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_005266390
          Length = 833

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TDAFLSTS 8
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_006719862
          Length = 848

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TDAFLSTS 8
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_005266389
          Length = 886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TDAFLSTS 8
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_005249758
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>XP_006715751
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 99  TDSFLTVSS 107
>XP_005249757
          Length = 886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>XP_006715750
          Length = 887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAFLSTST 9
           TD+FL+ S+
Sbjct: 190 TDSFLTVSS 198
>XP_005248537
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   DAFLSTS 8
           DAF STS
Sbjct: 310 DAFASTS 316
>XP_005248536
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   DAFLSTS 8
           DAF STS
Sbjct: 372 DAFASTS 378
>NP_057085
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TDAFLSTST 9
           T  F+STST
Sbjct: 221 TGNFISTST 229
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.110    0.274 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 363,062
Number of extensions: 636
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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